Effect of microemulsion formulation on biodistribution of ^99mTc-Aprotinin in acute pancreatitis models induced rats

Figures & data

Table 1. Rf values of ^99mTc-Aprotinin in mobile phases.

	Whatman 3MM	ITLC-SG^a
	Acetone	A/E/W
Free ^99mTc	0.7–1.0	0.7–1.0
R/H ^99mTc	0.0	0.0
^99mTc-Aprotinin	0.0	0.7-1.0

^aAlbumin impregnated.

Figure 1. Radiolabeling efficiency and stability of ^99mTc-Aprotinin at room temperature up to 6 h.

Figure 2. The scintigrams of ^99mTc-Aprotinin-S and ^99mTc-Aprotinin-M injected acute edematous, model induced rats at different time intervals up to 90 min (approximate organ regions for K: kidney, IS: injection site, L: liver, S: spleen).

Figure 3. The scintigrams of ^99mTc-Aprotinin-S and ^99mTc-Aprotinin-M injected severe necrotizing pancreatitis model induced rats at different time intervals up to 90 min (approximate organ regions for K: kidney, IS: injection site, L: liver, S: spleen).

Figure 4. The scintigrams of ^99mTc-Aprotinin-S and ^99mTc-Aprotinin-M injected air pouch model induced rats at different time intervals up to 90 min (approximate organ regions for K: kidney, IS: injection site, L: liver, S: spleen).

Figure 5. Calculated Organ/BG ratios of acute edematous (A), severe necrotizing pancreatitis (B) and air pouch model (C) induced rats after ^99mTc-Aprotinin-S injection up to 90 min.

Figure 6. Calculated organ/BG ratios of acute edematous (A), severe necrotizing pancreatitis (B) and air pouch model (C) induced rats after ^99mTc-Aprotinin-M injection up to 90 min.

Table 2. Biodistribution results of (organ/blood ratios) ^99mTc-Aprotinin-S and ^99mTc-Aprotinin-M injected acute edematous (A) induced rats at 90 min post-injection.

	Organ/blood uptake ratios
	^99mTc-Aprotinin-S	^99mTc-Aprotinin-M
Heart	0.407 ± 0.033	0.494 ± 0.106
Liver	0.847 ± 0.108	1.160 ± 0.012
Pancreas	0.752 ± 0.060	1.269 ± 0.018
Lung	0.612 ± 0.504	0.689 ± 0.008
Spleen	0.496 ± 0.019	0.520 ± 0.011
Kidney	9.484 ± 4.626	1.372 ± 0.764

Table 3. Biodistribution results of (organ/blood ratios) ^99mTc-Aprotinin-S and ^99mTc-Aprotinin-M injected severe necrotizing pancreatitis (B) induced rats at 90 min post-injection.

	Organ/blood uptake ratios
	^99mTc-Aprotinin-S	^99mTc-Aprotinin-M
Heart	0.438 ± 0.024	0.652 ± 0.047
Liver	0.976 ± 0.199	2.000 ± 0.016
Pancreas	0.472 ± 0.031	1.435 ± 0.013
Lung	0.899 ± 0.047	0.565 ± 0.007
Spleen	0.325 ± 0.026	0.826 ± 0.011
Kidney	2.662 ± 1.913	1.439 ± 0.719

Table 4. Biodistribution results of (organ/blood ratios) ^99mTc-Aprotinin-S and ^99mTc-Aprotinin-M injected air pouch model (C) induced rats at 90 min post-injection.

	Organ/blood uptake ratios
	^99mTc-Aprotinin-S	^99mTc-Aprotinin-M
Heart	0.483 ± 0.056	0.473 ± 0.011
Liver	0.814 ± 0.022	0.464 ± 0.011
Pancreas	0.331 ± 0.018	0.328 ± 0.144
Lung	0.246 ± 0.014	1.693 ± 0.019
Spleen	0.314 ± 0.060	0.579 ± 0.230
Kidney	7.58 ± 2.965	1.6740 ± 0.485