Relationship Between CX3CR1 Genetic Polymorphism and Carotid Atherosclerosis

Figures & data

Figure 1. PCR-RELP analysis of V249I CX3CR1 gene polymorphisms. Amplified PCR products were digested with Acl I enzyme.

Lane M, molecular marke, Lane 1, genotype VV, Lane 2, genotype II, Lane 3, genotype VI

Figure 2. PCR-RELP analysis of T280M CX3CR1 gene polymorphisms. Amplified PCR products were digested with HpyCH4 III enzyme.

Lane M, molecular marker, Lane 1,genotype MM, Lane 2, genotype TM, Lane 3,genotype TT

Table 1. Baseline characteristics in patients and controls

	Patients (318) n (%)	Controls (292) n (%)	χ^2	P
Male	189 (59)	182 (62)	0.421	0.517
Hypertension	102 (32)	63 (22)	7.981	0.005
Hypercholesterolemia	80 (25)	52 (18)	4.425	0.035
Diabetes mellitus	45 (14)	22 (8)	6.156	0.013
Smoking	52 (16)	24 (8)	8.501	0.004

Table 2. CX3CR1 genotype and allele frequencies in patients and controls

Group	V249I Genotype (n/%)			V249I Allele frequency (n/%)		T280M Genotype (n/%)			T280M Allele frequency (n/%)
	VI	II	VV	V	I	TM	MM	TT	T	M
Patients	66/21	58/18	194/61	454/71	182/29	56/18	16/5	246/77	548/86	88/14
Controls	65/22	63/22	164/56	393/67	191/33	70/24	21/7	201/69	472/81	112/19

VI+II vs VV, OR=1.012 (95%CI 0.731-1.403), p =0.940. V VSI, OR=0.825 (95%CI 0.646-1.053), p=0.121. TM+MM vs TT, OR = 0.646 (95%CI 0.451-0.928), p = 0.017. T VSM, OR = 0.677 (95%CI 0.499-0.918), p = 0.010.

Table 3. Combined genotype frequencies of V249I and T280M polymorphisms in two groups.

Combined Genotype	V249I	T280M	Patients n (%)	Controls n (%)
1	VV	TT	194 (61)	164 (56)
2	II	TT	23 (7)	12 (4)
3	II	MM	16 (5)	21 (7)
4	II	TM	19 (6)	30 (10)
5	VI	TT	29 (9)	25 (9)
6	VI	TM	37 (12)	40 (14)

Table 4. Multiple logistic regression analysis

Variables	OR (95% CI)	P
Smoking	1.682 (1.171 — 2.418)	0.005
Hypertension	1.553 (1.108 — 2.178)	0.011
Diabetes mellitus	1.245 (0.868—1.787)	0.234
Hypercholesterolemia	1.447 (1.034—2.025)	0.031
VI+II	0.948 (0.596—1.509)	0.823
TM+MM	0.426 (0.291 — 0.987)	0.042