Figures & data
Figure 1. Molecular distribution of crosslinked bovine stroma-free polyhemolysate. The elution profile of stroma-free polyhemolysate was obtained by running 1 ml of stroma-free polyhemolysate on Sepacryl S-300 1.5398cm column with 0.1 M Tris-Hcl, 0.15M NaCl pH 7.5 buffer at elution speed at 36 ml/hr.
Figure 2. Ouchterlony double diffusion test for determining antigen specific antibody activation. Equal amounts of stroma-free hemolysate (SFHb) (1g/dl) were placed into the central well of the 8% agarose gel plate (containing 2% sodium azide) and the same amount of serum from rat S1 (stroma-free hemolysate - SFHb injected) (positive control), rat P1 (purified stroma-free polyhemolysate - SFPolyHb injected) (test) and rat B1 (buffer injected) (negative control) were placed into two diagonal wells in duplicates, respectively (). The serum from rats S2, S3, P2, P3 and B2, B3 were repeated in the same way. We replaced stroma-free hemolysate - SFHb with stroma-free polyhemolysate into the central well and repeated the same procedure as above to determine anti-stroma-free polyhemolysate specific antibody production. All plates were incubated at room temperature in a humid environment for four days.
Table 1. Catalase activity of stroma-free hemolysate (SFHsate) and purified stroma-free polyhemolysate (SFPolyHsate)
Table 2. The plasma C3a des Arg level of rats immunized with buffer, stroma-free hemolysate (SFHsate), or stroma-free polyhemolysate (SFPolyHsate); the saline in each test was used as negative control
