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Research Article

Structural and Redox Behavior of OxyVita™, a Zero-linked Polymeric Hemoglobin: Comparison with Natural Acellular Polymeric Hemoglobins

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Pages 64-68 | Published online: 03 Mar 2010

Figures & data

Figure 1. Isothermal unfolding of acellular hemoglobins (Arenicola Hb, Lumbricus Hb, and OxyVita™, liquid and powder preparations) in the presence of urea at T = 37°C. All solutions were equilibrated for 30 minutes prior to spectral runs.

Figure 1. Isothermal unfolding of acellular hemoglobins (Arenicola Hb, Lumbricus Hb, and OxyVita™, liquid and powder preparations) in the presence of urea at T = 37°C. All solutions were equilibrated for 30 minutes prior to spectral runs.

Figure 2. Soret spectra of OxyVita™, liquid preparation (A), powder preparation (B), and Arenicola Hb (C) in urea solution (0-9 M urea) at T = 37°C.

Figure 2. Soret spectra of OxyVita™, liquid preparation (A), powder preparation (B), and Arenicola Hb (C) in urea solution (0-9 M urea) at T = 37°C.

Table 1. Free Energy of Unfolding(ΔG°u) in Absence of Denaturant and Unfolding Midpoint (D1/2) of Acellular Polymeric Hemoglobins T = 37°C

Figure 3. Changes in wavelength maximum within the Soret region for acellular hemoglobins (Arenicola Hb, Lumbricus Hb, and OxyVita™, liquid and powder preparations) in the presence of increasing concentrations of urea at T = 37°C.

Figure 3. Changes in wavelength maximum within the Soret region for acellular hemoglobins (Arenicola Hb, Lumbricus Hb, and OxyVita™, liquid and powder preparations) in the presence of increasing concentrations of urea at T = 37°C.

Table 2. Reduction (heme-Fe+3 heme-Fe+2) of Acellular Hbs by Ascorbic Acid, Glutathione and β-NADH T = 22°C, pH 7.0

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