Structural and Redox Behavior of OxyVita™, a Zero-linked Polymeric Hemoglobin: Comparison with Natural Acellular Polymeric Hemoglobins

John P. Harrington State University of New York, New Paltz, New York, USACorrespondence[email protected]

Kseniya Orlig State University of New York, New Paltz, New York, USA

Samantha L. Zito State University of New York, New Paltz, New York, USA

Jacek Wollocko OxyVita, Inc., New Windsor, New York, USA

Hanna Wollocko OxyVita, Inc., New Windsor, New York, USA

Figures & data

Figure 1. Isothermal unfolding of acellular hemoglobins (Arenicola Hb, Lumbricus Hb, and OxyVita™, liquid and powder preparations) in the presence of urea at T = 37°C. All solutions were equilibrated for 30 minutes prior to spectral runs.

Figure 2. Soret spectra of OxyVita™, liquid preparation (A), powder preparation (B), and Arenicola Hb (C) in urea solution (0-9 M urea) at T = 37°C.

Table 1. Free Energy of Unfolding(ΔG°u) in Absence of Denaturant and Unfolding Midpoint (D1/2) of Acellular Polymeric Hemoglobins T = 37°C

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Figure 3. Changes in wavelength maximum within the Soret region for acellular hemoglobins (Arenicola Hb, Lumbricus Hb, and OxyVita™, liquid and powder preparations) in the presence of increasing concentrations of urea at T = 37°C.

Table 2. Reduction (heme-Fe⁺³ heme-Fe⁺²) of Acellular Hbs by Ascorbic Acid, Glutathione and β-NADH T = 22°C, pH 7.0

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Structural and Redox Behavior of OxyVita™, a Zero-linked Polymeric Hemoglobin: Comparison with Natural Acellular Polymeric Hemoglobins

Table 1. Free Energy of Unfolding(ΔG°u) in Absence of Denaturant and Unfolding Midpoint (D1/2) of Acellular Polymeric Hemoglobins T = 37°C

Table 2. Reduction (heme-Fe⁺³ heme-Fe⁺²) of Acellular Hbs by Ascorbic Acid, Glutathione and β-NADH T = 22°C, pH 7.0

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Structural and Redox Behavior of OxyVita™, a Zero-linked Polymeric Hemoglobin: Comparison with Natural Acellular Polymeric Hemoglobins

Figures & data

Table 1. Free Energy of Unfolding(ΔG°u) in Absence of Denaturant and Unfolding Midpoint (D1/2) of Acellular Polymeric Hemoglobins T = 37°C

Table 2. Reduction (heme-Fe+3 heme-Fe+2) of Acellular Hbs by Ascorbic Acid, Glutathione and β-NADH T = 22°C, pH 7.0

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Table 2. Reduction (heme-Fe⁺³ heme-Fe⁺²) of Acellular Hbs by Ascorbic Acid, Glutathione and β-NADH T = 22°C, pH 7.0