Antigen-specific Immunoadsorption of Anti-acetylcholine Receptor Antibodies from Sera of Patients with Myastenia Gravis

Figures & data

Figure 1. Adsorption rates of anti-acetylcholine receptor antibodies (AChRAb) in the sera of patients with myasthenia gravis (MG) by the immunoadsorbent hMIR10-CB made by conjugation of 10 amino acids referring to the MIR at residues 67–76 of (α-subunit of human AChR and cellulose beads, and Trp-CB made by conjugation of tryptophan and cellulose beads.

Table 1. The effects of immunoadsorbent hMIR10-CB on blood cells and plasma ions

Cells and ions	Pre-adsorption	Post-adsorption	t	p
RBC (×1012/L)	5.18 ± 0.41	4.76 ± 0.20	2.916	0.062
WBC (×109/L)	5.60 ± 0.74	4.29 ± 2.06	0.963	0.407
HGB (g/L)	116.50 ± 5.26	112.25 ± 5.12	1.095	0.354
HCT (%)	39.90 ± 3.30	37.28 ± 6.22	1.680	0.191
PLT (×109/L)	62.25 ± 54.39	187.50 ± 59.79	2.240	0.111
K+ (mmol/L)	3.15 ± 0.30	3.25 ± 0.66	–0.473	0.609
Na+ (mmol/L)	129.25 ± 8.42	131.00 ± 14.79	–0.490	0.657
Cl+ (mmol/L)	108.75 ± 7.14	103.50 ± 6.95	2.414	0.095

RBC, red blood cell; WBC, white blood cell; HGB, hemoglobin; HCT, hematocrit; PLT, platelet.

Table 2. The changes in plasma proteins before and after adsorptions of immunoadsorbents hMIR10-CB and Trp-CB (mean± s, n = 5)

Plasma proteins	Trp-CB			hMIR10-CB
	Before (g/l)	After (g/l)	Adsorption Rate (%)	Before (g/l)	After (g/l)	Adsorption rate (%)
TP	60.50 ± 7.68	51.00 ± 9.42^a	16.1 ± 4.6	57.50 ± 2.52	46.50 ± 3.32^a	19.1 ± 5.8^b
ALB	36.25 ± 3.30	31.50 ± 3.70^a	13.2 ± 3.9	33.75 ± 2.63	29.50 ± 1.91^a	12.5 ± 2.1^b
GLO	24.25 ± 4.57	19.50 ± 6.40^a	21.0 ± 10.0	23.75 ± 1.89	6.75 ± 2.36^a	29.1 ± 11.2^b

TP, total protein; ALB: albumin; GLO: globulin; a, P < 0.05, changes of concentrations before and after adsorptions; b, P > 0.05, compared with Trp-CB group.