Extracorporeal Whole Blood Immuno-adsorption of Passively Transferred Myasthenia Gravis Rabbits by Cellulose-tryptophan Column

Figures & data

Figure 1. Schematic representation of WBIA (Yang L, 2005).

Table 1. Changes in blood components after 2 hrs WBIA

	Before WBIA	After WBIA	t	P
WBC(×10^9/l)	3.84 ±1.99	3.54±1.56	1.567	0.152
RBC(×10^12/l)	5.32±0.74	5.23 ±0.77	1.965	0.081
Hgb(g/l)	111.9±15.36	109.0±16.12	2.068	0.069
Hct(%)	30.42±1.83	29.91 ±2.04	1.67	0.129
Plt(×10^9/l)	327.2±98.22	324.9 ±98.03	1.793	0.107
TP(g/l)	56.9±4.56	49.7±6.22*	4.859	0.001
Alb(g/l)	28.2±2.29	27.3 ±2.71	1.536	0.159
Glo(g/l)	28.7±3.86	22.4±6.38*	3.735	0.005
Chol(mmol/l)	1.04±0.71	0.978±0.72	1.47	0.176
TG(mmol/l)	1.12±0.43	1.07±0.39	0.879	0.402
K^+ (mmol/l)	3.43±0.69	3.34±0.48	0.741	0.478
Na^+(mmol/l)	144.2±2.53	143.5±1.58	1.769	0.111
Cl^−(mmol/l)	99.9±4.51	97.5±3.21	1.616	0.14
Ca^2+(mmol/l)	2.59±0.27	2.53 ±0.23	1.727	0.118
CO2(mmol/l)	20.9 ±1.66	20.2±2.29	0.938	0.373

Table 2. Changes of antibodies removal rate in rabbits of the therapeutic group after 2 hrs WBIA

Time of WBIA (min)	Antibodies removal rate (%)
0	0
30	27.81±2.11
60	39.86±5.86
90	47.06±2.73
120	49.85+2.55

Table 3. Changes of blood antibodies OD value in rabbits of the therapeutic group after 2 hrs (492 nm) WBIA

Time of WBIA (min)	Anti-AChR antibodies (OD)
0	0.5389±0.1077
30	0.3843±0.0776
60	0.3240±0.0698
90	0.2846±0.0542
120	0.2690±0.0481

Table 4. Comparison of the decrement of RNS between the therapeutic group and the control group

Frequency	therapeutic group (%)		control group (%)
	D5	D8	D5	D8
3Hz	21.875±3.226	17.875 ±1.642	21.375±2.615	21.125±2.416
5Hz	22.250±2.815	18.750±1.388	21.875±2.232	22.000±1.772
10Hz	24.375 ±1.685	23.250±1.388	23.875±1.126	24.125 ±2.696

Table 5. Comparison of grading of clinical manifestation on the 5th day (D5) and 8th day (D8) after passive transfer between the therapeutic and the control group

Control group		Therapeutic group
D5	D8	D5	D8
+ +	+	+ +	+
+	+	+	0
+	+	+	0
+ +	+ +	+ +	+
+ + +	+ + +	+ + +	+ +
+	+	+	0
+ +	+ +	+ +	+
+ +	+ +	+ +	0

Figure 2. Neuromuscular junctions therapeutic group rabbits before WBIA (×200).

Figure 3. Neuromuscular junctions of the therapeutic group rabbits on the 3th day after WBIA (×200).