Analysis of Gelatin Plasma Substitutes in Blood Based on Detection of Hydroxyproline Derivatives

Figures & data

Figure 1. SEC-RI-MALLS detection of gelatin plasma substitutes. A: Succinylated gelatin; B: Genipin crosslinked gelatin.

Table 1. Physicochemical characteristics of gelatin plasma substitutes

Physicochemical characteristics	Succinylated gelatin	Genipin-crosslinked gelatin
Concentration (g/l)	40 (4%)	40 (4%)
Isoeletric point	4.5	4.6
Melting point	<4 °C	<4 °C
Relative viscosity (0.9% NaCl)	2.5	3.1
pH	7.4	7.4
Colloid osmotic pressure (mmHg)	33	29
Number of average molecular weight	25400	28500
Modification degree (30)	43%	–

Figure 2. Chromatograms of different free amino acids derivatives. 1: Aspartic acid; 2: Glutamic acid; 3: Hydroxyproline; 4: Serine; 5: Glycine; 6: Threonine; 7: Proline; 8: Alanine; 9: Valine; 10: Isoleucine; 11: Leucine; 12: Methionine; 13: Phenylalanine; 14: Histidine; 15: Lysine; 16: Tryptophane.

Figure 3. Chromatograms of hydroxyproline derivatives from blood/gelatin mixtures. Blood/gelatin ratio: A: 100:0; B: 100:2; C: 100:10; D: 100:20; E: 100:50; F: 100:100.

Figure 4. Relative content change of gelatin plasma substitutes in vivo after transfusion. A: Succinylated gelatin; B: Genipin crosslinking gelatin.

Table 2. Dynamic modeling parameters of succinylated and genipin crosslinked gelatin^a

Parameter	y0	A	α	B	β	R^2	t1/2 (h)
Succinylated gelatin	0.004	0.7154	0.05279	0.3729	0.9727	0.989	6.7
Genipin-crosslinked gelatin	0.004	0.3421	1.2095	0.7712	0.1380	0.996	3.3

^aEquation : y = y₀ + Ae^−αt + Be^−βt

Figure 5. Relative content change of amino acids in rabbit blood after transfusion of succinylated gelatin plasma substitute.