Figures & data
Figure 1. Standard curve of current (mA) vs. ascorbate (mg/dl) by ascorbate oxidase biosensor using epoxy resin membrane bound bottle gourd fruit ascorbate oxidase.
Figure 2. (a) SEM of epoxy resin ‘Araldite’ membrane without enzyme at 460 ×. (b) SEM of Araldite membrane with enzyme at 460 ×.
Figure 3. Schematic diagram of immobilization of enzyme (Ascorbate oxidase) onto epoxy resin ‘Araldite’ membrane mounted onto gold electrode (AuE).
Figure 4. Lineweaver-Burk plot for epoxy resin membrane bound bottle gourd fruit ascorbate oxidase.
Table 1. A comparison of ascorbate biosensors using plant ascorbate oxidase
Table 2. Analytical recovery of added ascorbate in serum, as measured by ascorbate biosensor using epoxy resin membrane bound bottle gourd fruit ascorbate oxidase
Table 3. Ascorbate level in sera of apparently healthy adults, as determined by ascorbate biosensor based on epoxy resin membrane bound bottle gourd fruit ascorbate oxidase
Figure 5. Correlation between serum ascorbate values as determined by standard DCPIP titration method (x-axis) and present biosensor (y-axis) based on epoxy resin membrane bound bottle gourd ascorbate oxidase.
Table 4. Determination of ascorbic acid in various fruit juices by amperometric ascorbate biosensor based on epoxy resin membrane bound bottle gourd fruit ascorbate oxidase
Table 5. Effect of various serum substances on response of ascorbate biosensor based on epoxy resin membrane bound bottle gourd fruit ascorbate oxidase