Basic Science Offers a Challenge for Developing Hemoglobin Based Oxygen Carriers into Therapeutic Agents

Figures & data

Figure 1. Comparison of the time course of the MAP increase produced by infusion in rats exchange transfused with solutions of either fumaryly X-linked bovine hemoglobin (a stabilized tetramer) or albumin with L-NAME. Ł) Albumin, Ł) Albumin + L-NAME, Ł) X-linked HB, Ł) L-NAME alone. Adapted from [5].

Figure 2. Effect of infusion of fumaryl X-linked bovine Hb (a stabilized tetramer) on the blood flow of various internal organ in the cat. Notably, heart and brain show an increased blood flow. Adapted from [6].

Table 1. Pores in the capillary walls (diameter).

Small pores 80Δ

Glomeruli pores 60-65Δ

Large pores 500-600Δ

Hemoglobin tetramers 60Δ

Hemoglobin dimers 48Δ

Adapted from [9]

Figure 3. In rats exchange transfused with ZL-HbBv polymer (average MW 25 MDa) the NO independent vasoconstriction reverts to vasodilation when PVP is added to circulating blood. In the albumin controls, PVP was much less effective, as if vasodilation were oxygen dependent. Adapted from [12].

Figure 4. Decrease of infarct volume, produced in mice by occlusion of the middle cerebral artery, after infusion of HBOCs with different P₅₀. DECA is human hemoglobin X-linked with sebacic acid (MW 64 KDa), Polytauro is a recombinant heptameric HB polymer (seven tetramers) enriched with surface SH groups (MW near 500 KDa). (Polytauro)n is a recombinant Hb polymer enriched with surface SH groups (Average MW 1000KDa). Adapted from [24].

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