Figures & data
Figure 1. The scheme of nano-thin membrane (one bilayer) on biological material built of polyelectrolyte layers including avidin binding incorporated in the layers’ biotin.
Figure 2. The FTIR spectrum of PLL/PEI membrane after 8-day culture.
Figure 3. Confocal visualization of Jurkat cells coated with one layer PLLbiot complexed with avidin FITC (×3000).
Figure 4. AFM image of Jurkat cell coated with PLLcxPEI shell, deposited on filtration membrane (Nanoscope V, Veeco Instruments, Inc., USA).
Figure 5. Fluorescence microscopy visualization of Jurkat cells coated with two bilayers, PLLcxPEI, after 8-day culture. Encapsulated cells exhibited retained dye calcein.
Figure 6. Evaluation of the mitochondrial activity in MTT test of encapsulated Jurkat cells during 8-day culture: The ratio of absorbance for cells coated with polyelectrolyte layers to the negative control absorbance. PLL/PEI – absorbance of cells encapsulated with double bilayer of PLL and PEI double bilayer; PLLcxPEI – absorbance of cells encapsulated with PLL with incorporated biotin complexed with avidin and PE with incorporated biotin; CONTROL- non-encapsulated cells’ absorbance.
Figure 7. Evaluation of the mitochondrial activity of encapsulated islets during 8-day culture in MTT test: The ratio of absorbance for cells coated with PE to the negative control absorbance. isletsPLL/PEI – absorbance of islets encapsulated within double bilayer of PLL and PEI; isletsPLLcxPEI – absorbance of islets encapsulated with double bilayer of PLL with incorporated biotin complexed with avidin and PEI with incorporated biotin; CONTROL – non-encapsulated islets’ absorbance.
