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Research Article

Microencapsulated Mammalian Cells for Simultaneous Production of VEGF165b and IFNα

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Pages 1-6 | Published online: 31 Jan 2012

Figures & data

Figure 1. Photomicrograph of alginate-poly-L-lysine-alginate microcapsules (A) APA microcapsules without cells (B) APA microcapsules containing co-encapsulated engineered cells producing IFNα and engineered cells producing VEGF165b.

Figure 1. Photomicrograph of alginate-poly-L-lysine-alginate microcapsules (A) APA microcapsules without cells (B) APA microcapsules containing co-encapsulated engineered cells producing IFNα and engineered cells producing VEGF165b.

Figure 2. The number of viable co-microencapsulated cells determined by MTS assay. The capsules contain VEGF165b producing HEK293 cells and IFNα producing HEK293 cells.

Figure 2. The number of viable co-microencapsulated cells determined by MTS assay. The capsules contain VEGF165b producing HEK293 cells and IFNα producing HEK293 cells.

Figure 3. Western blot analysis of human IFNα and human VEGF165b produced by co-encapsulated cells. (A) Production of IFNα (B) production of VEGF165b at various time periods

Figure 3. Western blot analysis of human IFNα and human VEGF165b produced by co-encapsulated cells. (A) Production of IFNα (B) production of VEGF165b at various time periods

Figure 4. The quantity of secreted IFNα and VEGF165b from co-encapsulated cells determined by ELISA.

Figure 4. The quantity of secreted IFNα and VEGF165b from co-encapsulated cells determined by ELISA.

Figure 5. The quantity of secreted IFNα and VEGF165b from non-encapsulated cells determined by ELISA.

Figure 5. The quantity of secreted IFNα and VEGF165b from non-encapsulated cells determined by ELISA.

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