Temperature Effects on Enzyme Activity of Chicken Liver Esterase Used in Calorimetric Biosensor

Figures & data

Figure 1. Temperature effects on relative enzyme activity of free and immobilized chicken liver esterase.

Figure 2. DSC scan curve of D113 resin at 10 °C /min.

Figure 3. Temperature effects on inhibition ratio of free and immobilized chicken liver esterase: (a) free esterase; (b) adsorption immobilized esterase; (c) adsorption-microcapsule immobilized esterase; (d) adsorption-crosslinking immobilized esterase.

Table 1. Temperature effects on enzyme activity retention ratio (EARR) for immobilized esterase in operation.

	adsorption			adsorption-microcapsule			adsorption-crosslinking
	EA1	EA2	EARR	EA1	EA2	EARR	EA1	EA2	EARR
30°C	1.978 ± 0.03	1.414 ± 0.004	71.52 ± 1.08	1.376 ± 0.009	1.316 ± 0.011	95.65 ± 1.36	1.485 ± 0.011	1.436 ± 0.018	96.68 ± 1.91
40°C	2.084 ± 0.03	1.451 ± 0.011	69.67 ± 0.49	1.386 ± 0.043	1.322 ± 0.008	95.48 ± 3.3	1.495 ± 0.022	1.45 ± 0.008	97 ± 1.14
50°C	2.027 ± 0.04	1.426 ± 0.004	70.36 ± 1.59	1.386 ± 0.035	1.333 ± 0.011	96.21 ± 1.97	1.496 ± 0.011	1.448 ± 0.034	96.78 ± 1.7
60°C	1.985 ± 0.057	1.438 ± 0.011	72.49 ± 1.9	1.352 ± 0.09	1.318 ± 0.005	97.43 ± 0.99	1.478 ± 0.036	1.428 ± 0.011	96.6 ± 1.07

EA₁ indicate enzyme activity before operation (U/g); EA₂ indicate enzyme activity after operation (U/g).

Figure 4. The enzyme activity retention ratio (EARR) of free and immobilized chicken liver esterase at different temperature in storage: (a) 30°C; (b) 4°C; (c) −18°C; (d) −178°C.