Figures & data
Figure 5. The effect of pH on lysozyme adsorption: lysozyme concentration: 1.0 mg/mL; incubation time: 2 h; temperature: 25°C.
![Figure 5. The effect of pH on lysozyme adsorption: lysozyme concentration: 1.0 mg/mL; incubation time: 2 h; temperature: 25°C.](/cms/asset/c8998b64-badc-4f24-be6d-9630c1921895/ianb19_a_657204_f0005_b.gif)
Figure 6. Effect of concentration of lysozyme on the lysozyme adsorption; incubation time: 2 h; pH: 10.0; temperature: 25°C.
![Figure 6. Effect of concentration of lysozyme on the lysozyme adsorption; incubation time: 2 h; pH: 10.0; temperature: 25°C.](/cms/asset/64b59958-3452-4620-b456-a58b34110dde/ianb19_a_657204_f0006_b.gif)
Figure 7. Effect of salt type and concentration on lysozyme adsorption; lysozyme concentration: 0.5 mg/mL; incubation time: 2 h; pH: 10.0; temperature: 25°C.
![Figure 7. Effect of salt type and concentration on lysozyme adsorption; lysozyme concentration: 0.5 mg/mL; incubation time: 2 h; pH: 10.0; temperature: 25°C.](/cms/asset/bb301107-0a20-458b-8a42-7f15497c11ad/ianb19_a_657204_f0007_b.gif)
Figure 8. Effect of temperature on lysozyme adsorption; lysozyme concentration: 0.5 mg/mL; incubation time: 2 h; pH: 10.0.
![Figure 8. Effect of temperature on lysozyme adsorption; lysozyme concentration: 0.5 mg/mL; incubation time: 2 h; pH: 10.0.](/cms/asset/802217ae-211f-4872-943e-d0d3756466ac/ianb19_a_657204_f0008_b.gif)
Figure 9. Effect of time on lysozyme adsorption; lysozyme concentration: 1.0 mg/mL; incubation time: 2 h; pH: 10.0; temperature: 25°C
![Figure 9. Effect of time on lysozyme adsorption; lysozyme concentration: 1.0 mg/mL; incubation time: 2 h; pH: 10.0; temperature: 25°C](/cms/asset/0794132a-856c-4c36-a924-93ecc0058e70/ianb19_a_657204_f0009_b.gif)
Table I. Pseudo-first and second-order kinetic constants determined for nano-MIP and nano-NIP.
Figure 10. Adsorption capacities of the nano-MIP and nano-NIP for lysozyme, BSA, and Cyt-c. Adsorption time: 2 h; T: 20°C.
![Figure 10. Adsorption capacities of the nano-MIP and nano-NIP for lysozyme, BSA, and Cyt-c. Adsorption time: 2 h; T: 20°C.](/cms/asset/0b5c9313-4e1c-4591-9441-af4fefcc41a7/ianb19_a_657204_f0010_b.jpg)
Table II. The distribution (Kd), selectivity (k), and relative selectivity (k′) coefficient values of BSA and Cytochrome-c with respect to lysozyme.
Figure 11. SDS-PAGE of egg white fractions. The fractions were assayed by SDS-PAGE using 10% separating gel (9 × 7.5 cm), and 5% stacking gels were stained with 0.25% (w/v) Coomassie Brillant R 250 in acetic acid–methanol–water (1:5:5, v/v/v) and destained in ethanol–acetic acid–water (1:4:6, v/v/v). Lane 1, egg white before adsorption; lane 2, egg white after adsorption; lane 3, commercial lyzoyme; lane 4, commercial marker; lane 5, desorbed lysozyme. Equal amounts of sample were applied to each line.
![Figure 11. SDS-PAGE of egg white fractions. The fractions were assayed by SDS-PAGE using 10% separating gel (9 × 7.5 cm), and 5% stacking gels were stained with 0.25% (w/v) Coomassie Brillant R 250 in acetic acid–methanol–water (1:5:5, v/v/v) and destained in ethanol–acetic acid–water (1:4:6, v/v/v). Lane 1, egg white before adsorption; lane 2, egg white after adsorption; lane 3, commercial lyzoyme; lane 4, commercial marker; lane 5, desorbed lysozyme. Equal amounts of sample were applied to each line.](/cms/asset/aa935d94-f917-4f36-832e-35f74627ac27/ianb19_a_657204_f0011_b.jpg)