Synthesis of microwave-assisted poly(methyl vinyl ether-co-maleic anhydride)-bovine serum albumin bioconjugates

Figures & data

Scheme 1. Microwave-assisted covalent conjugation reaction scheme of P(MVE-MA)-BSA.

Table I. The experimental ratios and concentrations of n_BSA/n_P(MVE-MA) [BSA Mw:66 kDa and P(MVE-MA) Mw:70 kDa].

Ratios	The amount of BSA (mg)	The amount of P(MVE-MA) (mg)	Total PBS solutions (ml)
0.25	0.94	1	3.9
0.5	1.88	1	3.9
1	3.76	1	3.9
3	11.28	1	3.9
5	18.80	1	3.9

Figure 1. Conventional Method Conjugates (CMC): HPLC chromatograms of BSA, P(MVE-MA), and BSA-P(MVE-MA) conjugates prepared at ratio on n_BSA/n_P(MVE-MA): 0.25(1), 0.5(2), 1(3), 3(4), 5(5).

Figure 2. Microwave Assisted Conjugates (MWC): HPLC chromatograms of BSA, P(MVE-MA), and BSA-P(MVE-MA) conjugates prepared at ratio on n_BSA/n_P(MVE-MA): 0.25(1), 0.5(2), 1(3), 3(4), 5(5).

Figure 3. SEC [UV (a), LS (b)] chromatograms of the conjugates by conventional method of BSA-P(MVE-MA) bioconjugates prepared at the ratios of n_BSA/n_P(MVE-MA): 0.25(1), 0.5(2), 1(3), 3(4), 5(5).

Figure 4. SEC [UV (a), LS (b)] chromatograms of the conjugates by means of the microwave-assisted method of BSA-P(MVE-MA) bioconjugates prepared at the ratios of n_BSA/n_P(MVE-MA): 0.25(1), 0.5(2), 1(3), 3(4), 5(5).

Figure 5. The area of the UV of the conjugates (MWC) (1) and conjugates (CMC) (2) prepared at the ratios of n_BSA/n_P(MVE-MA): 0.25, 0.5, 1, 3, 5.

Figure 6. The area of the LS of the conjugates (MWC) (1) and conjugates (CMC) (2) prepared at the ratios of n_BSA/n_P(MVE-MA): 0.25, 0.5, 1, 3, 5.