Nanomaterial-based composite biosensor for glucose detection in alcoholic beverages

Figures & data

Table I. Recovery data of glucose detection in alcoholic beverages obtained with the Au-np/GOx/GCPE by using chronoamperometric method.

Samples	Recovery (%, n = 3)
Red Wine	% 105.3
White Wine	% 100.0
Draft Beer	% 96.4
Mint Liqueur	% 105.3

Figure 1. The effect of enzyme amount for 400 µM glucose on the biosensor response at various amounts of enzyme: background; 39.8 unit; 47.8 unit; 55.7 unit; 71.6 unit; 79.6 unit; conditions: phosphate buffer solution (50 mM, pH 7.5), applied potential + 0.9 V, 2 µL Au-np.

Figure 2. The effect of Au-np amount for 400 µM glucose on the biosensor response at various amounts of Au-np: background; 0.5 µL; 1 µL; 2 µL; 5 µL; 10 µL; conditions: phosphate buffer solution (50 mM, pH 7.5), applied potential + 0.9 V, 55.7 unit GOx.

Figure 3. Chronoamperometric responses of 400 µM glucose on the biosensor response at various pH: (a) background; (b) 6.0; (c) 6.5; (d) 7.0; (e) 7.5; (f) 8.0; conditions: phosphate buffer solution system, applied potential + 0.9 V, 55.7 unit GOx, 2 µL Au-np; Inset: the effect of optimum pH curve of Au-np/GOx/GCPE.

Figure 4. The effect of applied potential for 400 µM glucose on the biosensor response at different potentials: 0.5 V; 0.6 V; 0.7 V; 0.8 V; 0.9 V; 1.0 V; conditions: phosphate buffer solution (50 mM, pH 7.5), 2 µL Au-np, 55.7 unit enzyme.

Figure 5. Calibration graph for glucose at Au-np/GOx/GCPE conditions: in 50 mM phosphate buffer solution (pH 7.5) and working potential + 0.8 V, 25°C.