Effects of agitation speed on the ex vivo expansion of cord blood hematopoietic stem/progenitor cells in stirred suspension culture

Figures & data

Figure 1. The flow field in culture flasks at different agitation speeds. A: 30 rpm, B: 45 rpm, C: 60 rpm, D: 80 rpm.

Figure 2. Expansion of total cell at different agitation speeds. *: P < 0.05 vs Control; **: P < 0.01 vs Control.

Table 1. CFC density in the culture at different agitation speeds.

Culture time (day)	Control (/10^5)	30 rpm (/10^5)	45 rpm (/10^5)	60 rpm (/10^5)	80 rpm (/10^5)
0	105.3 ± 12.7	105.3 ± 12.7	105.3 ± 12.7	105.3 ± 12.7	105.3 ± 12.7
3	112.7 ± 18.6	133.8 ± 23.1	124.5 ± 19.8	47.3 ± 12.1**	12.8 ± 5.3**
6	143.6 ± 23.9	195.2 ± 24.6^*	135.8 ± 21.4	21.9 ± 4**	2.4 ± 0.5**
9	88.2 ± 10.6	71 ± 18.7	45.6 ± 11.8*	5.6 ± 0.8**	0**
12	43.4 ± 9.3	22.1 ± 6.9**	12.5 ± 6**	0**	0**
15	25.1 ± 7.4	13.6 ± 5.1*	9.3 ± 4.4**	0**	0**

*: P < 0.05 vs Control; **: P < 0.01 vs Control.

Table 2. CD34 ⁺ cell population in the culture at different agitation speeds.

Culture time (day)	Control (%)	30 rpm (%)	45 rpm (%)	60 rpm (%)	80 rpm (%)
0	1.26 ± 0.17	1.26 ± 0.17	1.26 ± 0.17	1.26 ± 0.17	1.26 ± 0.17
3	2.24 ± 0.42	2.21 ± 0.66	1.78 ± 0.32	1.80 ± 0.38	1.26 ± 0.51*
6	2.65 ± 0.31	2.52 ± 0.57	2.37 ± 0.51	2.13 ± 0.24*	1.39 ± 0.19**
9	3.08 ± 0.43	3.01 ± 0.26	1.57 ± 0.39*	1.05 ± 0.36**	0.87 ± 0.16*
12	0.87 ± 0.18	1.23 ± 0.34	0.69 ± 1.43	0**	0**
15	0.40 ± 0.08	0.47 ± 0.11	0.39 ± 0.07	0**	0**

*: P < 0.05 vs Control; **: P < 0.01 vs Control.

Figure 3. CD34 ⁺ cells expansion at different agitation speeds. *: P < 0.05 vs Control; **: P < 0.01 vs Control.