The fabrication of nanosensor-based surface plasmon resonance for IgG detection

Figures & data

Figure 1. Size distribution of PHEMA nanoparticles.

Figure 2. TEM image of PHEMA nanoparticles.

Figure 3. FTIR spectra of IMEO, PHEMA nanoparticles, and PHEMA/IMEO nanoparticles.

Figure 4. AFM images of SPR chip surface.

Figure 5. Kinetic studies with PHEMA/IMEO nanoparticles attached on SPR chip.

Table I. Kinetic and isotherm parameters for PHEMA/IMEO nanoparticles–attached SPR sensor.

Association kinetic analysis				Scatchard
ka, μg/mL.s	0.04			ΔRmax, reflectivity%	2.30
kd, 1/s	0.0215			KA, µg/mL	2.624
KA, µg/mL	1.810			KD, mL/µg	0.381
KD, mL/µg	0.53			R^2	0.9285
R^2	0.9274
Langmuir		Freundlich		Langmuir–Freundlich
ΔRmax, reflectivity%	2.8161	ΔRmax, reflectivity%	2.186	ΔRmax, reflectivity%	0.264
KA, µg/mL	2.999	1/n	0.5226	1/n	3.288
KD, mL/µg	0.334	R^2	0.9423	KA, µg/mL	1.107
R^2	0.9776			KD, mL/µg	0.9035
				R^2	0.8594

Table II. Selectivity coefficients (SC) of PHEMA nanoparticles–attached SPR chip for IgG molecules.

Protein	ΔR	SC, ΔRIgG/ΔRcompetitor
IgG	2.1253	–
Albumin	0.4982	4.2659
Hemoglobin	0.8975	2.3680

Figure 6. Selectivity and specificity studies of modified SPR chip.

Figure 7. SPR chip response in human serum.