Preparation and in vitro percutaneous penetration of simvastatin ethosome gel

Figures & data

Table I. Prescriptions of simvastatin gel.

Prescription	I	II	III	IV
Simvastatin ethosome	0	0	0	40 ml
Simvastatin	0.06 g	0.06 g	0.06 g	0
Carbomer	0.5 g	0.5 g	0.5 g	0.5 g
Glycerol	0.5 g	0.5 g	0.5 g	0.5 g
Menthol	0	0.5 g	1.5 g	0
Ethanol （75%）	30 ml	30 ml	30 ml	0
Distilled water	To 50 g	To 50 g	To 50 g	To 50 g

Figure 1. Percutaneous permeation device.

Figure 2. Cumulative permeation amount per unit area.

Table II. Cumulative amounts of different preparations at different times (Character± s, n = 3).

Time (h)	Preparation I (μg ml−^1)	Preparation II (μg ml−^1)	preparation III (μg ml−^1)	Preparation IV (μg ml−^1)
2	1.70 ± 0.34	2.44 ± 0.48	2.46 ± 0.57	2.55 ± 0.43
4	3.35 ± 1.04	4.78 ± 0.66	5.47 ± 0.75*	7.74 ± 1.01*^＃▲
6	5.14 ± 0.81	6.84 ± 0.80	8.42 ± 1.17*	11.13 ± 1.12*^＃▲
8	6.57 ± 0.98	10.65 ± 1.07*	12.78 ± 0.68*^＃	17.25 ± 1.10*^＃▲
12	7.62 ± 0.90	12.81 ± 1.21*	16.04 ± 1.27*^＃	20.70 ± 0.89*^＃▲
24	9.40 ± 0.84	15.87 ± 0.72*	18.87 ± 1.13*	23.15 ± 0.96*^＃▲

*Compared with preparation I, P < 0.05; ＃ Compared with Preparation II, P < 0.05; ▲ Compared with Preparation Ⅲ, P< 0.05;

Table III. Simvastatin retention in mouse skin.

	Retention amount (μg ml−^1)	x-± s (μg·ml−^1)	RSD (%)
Preparation I	15.19
	15.54	16.07 ± 1.24	0.08
	17.48
Preparation II	30.01
	28.81	29.32 ± 0.62*	0.02
	29.13
Preparation III	25.39
	34.85	30.29 ± 4.74*	0.15
Preparation IV	30.61
	27.26
	43.67	34.57 ± 8.35*	0.24
	32.77

Compared with preparation I, P < 0.05.

Figure 3. Simvastatin retention in mouse skin.