Methylated arginines and nitric oxide in end-stage renal disease: impact of inflammation, oxidative stress and haemodialysis

Abstract

Objectives: To determine whether inflammation (C-reactive protein, CRP), oxidative stress (malondialdehyde, MDA) or haemodialysis (HD) affect associations between asymmetric (ADMA), symmetric (SDMA) dimethylarginine, NG-monomethyl-L-arginine (L-NMMA) and nitrite/nitrate (NOx) in end-stage renal disease (ESRD).

Method: Metabolites were measured pre-HD, after 1 hour and end-HD in 40 ESRD patients (age 63 ± 14 years).

Results: Positive associations between NOx and ADMA (p = 0.04), SDMA (p < 0.001) and L-NMMA (p = 0.04) were observed pre-HD. Associations weakened during HD but were not significantly influenced by CRP or MDA.

Conclusions: HD, oxidative stress or inflammation did not significantly affect the positive associations between methylated arginines and NOx in ESRD.

• Cardiovascular disease
• metabol(n)omics
• renal disease

Acknowledgements

We wish to thank the dialysis patients at Flinders Medical and Hartley Dialysis Centres for participating in this study. A. A. M. and N. B. conceived the study; C. H., J. A. B. and A. A. M. collected the data; N. B., R. J. M., E. D. and A. A. M. analysed and interpreted the data; N. B. wrote the first manuscript draft; all authors critically reviewed the original draft and subsequent versions of the article.