Associations of polymorphisms in adiponectin and leptin genes with men's longevity

Figures & data

Table I.  Primers sequences, PCR conditions and restriction enzymes used for genotyping of SNPs.

SNP site	Primers	Annealing temperature	Restriction enzyme	Fragment length
ADIPOQ
rs266729	F: 5′-AAGAACCGGCTCAGATCCTGC-3′	58	HinP1I (37°C)	CC → Wt 120
	R: 5′-GCTGCCACCCACTTAGGTGTTC-3′			GG → Mt 100, 20
rs2241766	F: 5′-CTGAGATGGACGGAGTCC TTT-3′	58	SmaI (25°C)	TT → Wt 456
	R: 5′-CCAAATCACTTCAGGTTGCTT-3′			GG → Mt 313, 143
rs1501299	F: 5′-GTCTAGGCCTTAGTTAATAATGAATG-3′	58	BsmI (37°C)	GG → Wt 82, 22
	R: 5′-GAGAAAGGAGATCCAGGTAAGA-3′			TT → Mt 104
LEP
rs7799039	F: 5′-TTTCCTGTAATTTTCCCGTGA-3′	52	HhaI (37°C)	AA → Wt 242
	R: 5′-AAAGCAAAGACAGGCATAAAA-3′			CC → Mt 181, 61

Table II.  Frequencies of ADIPOQ and LEP alleles and genotypes in control and elderly groups.

Genotypes and Alleles	Control group, n (%)	Elderly group, n (%)	p value
rs2241766 ADIPOQ*
TT	68 (56.6)	70 (63.6)
TG	43 (35.8)	37 (33.6)	0.231^†
GG	9 (7.6)	3 (2.8)
Allele T	179 (74.6)	177 (80.5)	0.164^‡
Allele G	61 ( 25.4)	43 (19.5)
rs1501299 ADIPOQ*
GG	49 (40.8)	58 (52.7)
GT	56 (46.6)	43 (39.1)	0.172^‡
TT	15 (12.6)	9 (8.2)
Allele G	154 (64.2)	159 (72.3)	0.078^‡
Allele T	86 (35.8)	61 (27.7)
rs266729 ADIPOQ*
CC	69 (57.5)	68 (61.8)
CG	43 (35.8)	35 (31.8)	0.809^‡
GG	8 (6.7)	7 (6.4)
Allele C	181 (75.4)	171 (77.7)	0.635^‡
Allele G	59 (24.6 )	49 (22.3)
rs7799039 LEP*
AA	29 (24.2)	34 (30.9)
AC	53 (44.2)	55 (50)	0.082^‡
CC	38 (31.6)	21 (19.1)
Allele A	111 (46.3)	123 (55.9)	0.051^‡
Allele C	129 ( 53.7)	97 (44.1 )

*All groups were in Hardy–Weinberg equilibrium (p > 0.05).

^†Fisher Exact test.

^‡Chi Square test.

Table III.  Frequencies of ADIPQO and LEP alleles and genotypes in control and elderly men subjects.

Genotypes and alleles	Control group, n (%)	Elderly group, n (%)	p value
rs2241766 ADIPQO*
TT	40 (56.3)	41 (60.3)
TG	27 (38.0)	25 (36.8)	0.799^†
GG	4 (5.7)	2 (2.9)
Allele T	107 (75.4)	107 (78.6)	0.606^‡
Allele G	35 (24.6 )	29 (21.4)
rs1501299 ADIPQO*
GG	21 (29.6)	37 (54.4)
GT	42 (59.2)	27 (39.7)	0.011^†
TT	8 (11.2)	4 (5.9)
Allele G	84 (59.2)	101 (74.3)	0.011^‡
Allele T	58 (40.8)	35 (25.7)
rs266729 ADIPQO*
CC	48 (67.6)	43 (63.2)
CG	19 (26.8)	23 (33.8)	0.509^†
GG	4 (5.6)	2 (3.0)
Allele C	115 (81.0)	109 (80.1)	0.980^‡
Allele G	27 ( 19.0)	27 (19.9)
rs7799039 LEP*
AA	16 (22.5)	23 (33.8)
AC	29 (40.8)	34 (50.0)	0.021^‡
CC	26 (36.6)	11 (16.2)
Allele A	61 (43.0)	80 (58.8)	0.011^‡
Allele C	81 (57.0 )	56 ( 41.2)

*All groups were in Hardy–Weinberg equilibrium (p > 0.05).

^†Fisher Exact test.

^‡Chi Square test.

Table IV.  Frequencies of ADIPOQ and LEP alleles and genotypes in control and elderly women subjects.

Genotypes and alleles	Control group, n (%)	Elderly group, n (%)	p value
rs2241766 ADIPQO*
TT	28 (57.1)	29 (69.0)
TG	16 (32.7)	12 (28.6)	0.266^†
GG	5 (10.2)	1 (2.4)
Allele T	72 (73.5)	70 (83.3)	0.115^‡
Allele G	26 (26.5)	14 (16.7)
rs1501299 ADIPQO*
GG	28 (57.1)	21 (50.0)
GT	14 (28.6)	26 (31.0)	0.788^‡
TT	7 (14.3)	5 (11.9)
Allele G	70 (71.4)	58 (69.0)	0.621^‡
Allele T	28 (28.6)	26 (31.0)
rs266729 ADIPQO*
CC	21 (42.9)	25 (59.5)
CG	24 (49.0)	12 (28.5)	0.145^†
GG	4 (8.1)	5 (12.0)
Allele C	66 (67.3)	62 (73.8)	0.430^‡
Allele G	32 (32.7)	22 (26.2)
rs7799039 LEP*
AA	13 (26.5)	11 (26.2)
AC	24 (48.9)	21 (50.0)	0.962^‡
CC	12 (24.4)	10 (23.8)
Allele A	50 (51.0)	43 (51.2)	0.990^‡
Allele C	48 (49.0)	41 (48.8)

*All groups were in Hardy–Weinberg equilibrium (p > 0.05).

^†Fisher Exact test.

^‡Chi Square test.