Quercetin-modulated erythrocyte membrane sodium-hydrogen exchanger during human aging: correlation with ATPase’s

Abstract

Content: Quercetin uptake by erythrocytes is rapid. The sodium-hydrogen exchanger (NHE) is a secondary active transporter, regulating intracellular pH, Na⁺ concentration and cell volume. Objective: The aim of present study was to investigate NHE as a function of human age and effect of quercetin on its activity. The NHE activity was correlated with erythrocytes ATPases. Materials and methods: We analyzed normal, healthy subjects of both sexes (20–82 years). NHE activity was estimated in terms of amiloride-sensitive H+-efflux from acid-loaded cells. Results: A significant age-dependent increase in NHE activity was observed during aging in humans. Concentration (10⁻³M to 10⁻⁸M)-dependent in vitro treatment with quercetin causes inhibition of NHE activity. The Na⁺/K⁺ -ATPase (r = 0.8882) and Ca²⁺-ATPase (r = 0.9540) activities positively correlated with it. Discussion and conclusion: The present data show an additional mechanism where dietary flavonoids may exerts beneficial effect during aging.

• Aging
• Ca²⁺-ATPase
• Na⁺/H⁺ exchanger
• Na⁺/K⁺ -ATPase
• quercetin

Acknowledgements

Pawan Kumar Maurya acknowledges support by fellowship (Science without Borders- Level A) from the coordination of Improvement of Higher Education Personnel (CAPES), National Counsel of Technological and Scientific Development (CNPq), Brazil. Pawan Kumar Maurya and Prabhanshu Kumar thanks Amity University Uttar Pradesh, Noida for providing necessary facility for the completion of this research work.

Declaration of interest

The authors report no conflicts of interest.