Investigation of the methanol root extract of Cochlospermum planchonii for pharmacological activities in vitro and in vivo

Figures & data

Figure 1.  Effect of the methanol root extract of C. planchonii on pentobarbital-induced sleeping time. *Significant at p <0.05 compared with the control group.

Table 1.  Effect of the methanol root extract of C. planchonii on acetic acid-induced writhing (analgesic test).

Group	Dose (mg/kg bw, p.o.)	Mean abdominal writhings ± SEM	% Inhibition
A Dist. water	10 (mL/kg bw)	78.6 ± 6.38	0
B ASA	400	44.8 ± 3.51*	43
C Extract	250	27.4 ± 4.49*	65.14
D Extract	500	20.4 ± 4.31*	74.05
E Extract	1000	49.8 ± 3.51*	35.16

*Significant difference at P<0.05 (ANOVA) compared with the untreated group; bw, body weight; p.o., per os; SEM, standard error of the mean; and ASA, acetyl salicylic acid.

Table 2.  Effect of the methanol root extract of C. planchonii on formalin (analgesic) test.

Group	Dose (mg/kg bw, p.o.)	PLT (mean ± SEM) seconds
		0-5 min	25-30 min
A Dist. water	10 (mL/kg bw, p.o.)	15.5 ± 2.55	25.6 ± 10.33
B ASA	400	47.4 ± 5.07*	31.2 ± 12.61
C Extract	250	19.4 ± 4.75	15.4 ± 7.29*
D Extract	500	33.8 ± 16.32*	13.4 ± 6.06*
E Extract	1000	31 ± 10*	13.4 ± 6.06*

*Significant difference at P <0.05 (ANOVA) compared with the untreated group; bw, body weight; p.o., per os; SEM, standard error of the mean; ASA, acetyl salicylic acid; PLT, paw licking time.

Table 3.  Effect of the methanol root extract of C. planchonii on leptazole-induced convulsions.

Treatment group		Duration of convulsion(min)	% mortality
A	Pentobarbital (35 mg/kg bw, i.p.) + Leptazole (90 mg/kg bw, i.p.)	18.6 ± 14.62	0
B	Extract (1, 000 mg/kg bw p.o.) + 45 min + Leptazole (90 mg/kg bw, i.p.)	37 ± 11.84	100
C	Leptazole (90 mg/kg bw, i.p.)	37.8 ± 9.56	100

Figure 2.  Effect of the methanol root extract of C. planchonii in carrageenan-induced paw edema in rats (anti-inflammatory test). CP, C. planchonii extract, p.o., per os.

Figure 3.  Antihyperglycemic effect of the methanol root extract of C. planchonii in alloxan-induce hyperglycemic mice. Significant at p < 0.05 with the control group; CP, C. planchonii extract; p.o., per os.

Table 4.  Effect of the methanol root extract of C. planchonii on blood glucose level in normoglycemic rats.

Group	Normal blood glucose level (mean ± SEM) mg%
	0 min	60 min	120 min	180 min	360 min
I Dist. water (10 mL/kg bw)	74.21 ± 5.50	81.34 ± 3.98	84.94 ± 10.77	82.58 ± 9.36	78.54 ± 12.64
II glibenclamide (2.5 mg/kg bw)	67.10 ± 2.56	62.75 ± 5.32	64.23 ± 2.21	61.98 ± 9.99	60.98 ± 12.74
III C. planchonii (250 mg/kg bw)	60.45 ± 7.89	67.38 ± 8.92	65.34 ± 5.34	62.69 ± 7.93	64.44 ± 5.67
IV C. planchonii (250 mg/kg bw)	90.14 ± 8.18	94.67 ± 10.91	85.45 ± 9.34	91.99 ± 21.78	85.56 ± 21.55
V C. planchonii (1000 mg/kg bw)	87.43 ± 10.76	85.42 ± 11.19	91.98 ± 19.87	80.48 ± 9.77	85.99 ± 1.65

*Significant at p <0.05 compared with the control group.