Antidiabetic and antioxidant activity of the methanol extract of Diospyros peregrina fruit on Type I diabetic rats

Figures & data

Table 1.  Effect of methanol extract of Diospyros peregrina on blood glucose level after single dose treatment.

Group ( n = 6)	Treatment	Blood glucose (mg/dL)
		0 h	2 h	6 h	12 h
I	Normal control (Distilled water)	70.67 ± 2.11	71 83 ± 3.23	70.83 ± 3.12	69.83 ± 2.29
II	Diabetic control (Distilled water)	253.83 ± 5.23^a	256.17 ± 8.59^a	256.67 ± 8.29^a	259.83 ± 8.85^a
III	Diabetic + DPME (150 mg/kg)	258.83 ± 4.35	223.5 ± 6.2^b	213.67 ± 7.61^b	210.67 ± 8.01^b
IV	Diabetic + DPME (300 mg/kg)	267.17 ± 5.68	216.17 ± 5.4^b	209.17 ± 5.74^b	206.83 ± 4.73^b
V	Diabetic + Glibenclamide (1 mg/kg)	275.83 ± 5.19	202.33 ± 8.76^b	193.5 ± 5.69^b	188.67 ± 3.9^b

Values are expressed as mean ± SE.

^ap < 0.001 compared with normal control group.

^bp < 0.01 compared with diabetic control group.

Table 2.  Effect of methanol extract of Diospyros peregrina on fasting blood glucose level.

Group ( n = 6)	Treatment	Blood glucose (mg/dL)
		0 day	1 day	6 day	12 day
I	Normal control (Distilled water)	70.67 ± 2.11	69.33 ± 2.03	72.17 ± 2.17	71.33 ± 1.98
II	Diabetic control (Distilled water)	253.83 ± 5.23^a	263.5 ± 8.12^a	272.17 ± 7.69^a	275.83 ± 5.01^a
III	Diabetic + DPME (150 mg/ kg)	258.83 ± 4.35	209.17 ± 6.89^b	188.83 ± 5.14^b	128.33 ± 3.24^b
IV	Diabetic + DPME (300 mg/ kg)	267.17 ± 5.68	203.17 ± 3.54^b	174.5 ± 6.19^b	118.5 ± 4.15^b
V	Diabetic + Glibenclamide (1 mg/kg)	275.83 ± 5.19	187.83 ± 3.02^b	166.17 ± 8.32^b	108.67 ± 3.21^b

Values are expressed as mean ± SE.

^ap < 0.001 compared with normal control group.

^bp < 0.01 compared with diabetic control group.

Table 3.  Effect of methanol extract of Diospyros peregrina on tissue TBARS, HP, GSH, SOD, and CAT in diabetic rats.

Parameters	Normal control (Distilled water)	Diabetic control (Distilled water)	Diabetic + DPME (150 mg/ kg)	Diabetic + DPME (300 mg/ kg)	Diabetic + Gliben clamide (1 mg/kg)
TBARS- Liver *	0.87 ± 0.14	1.55 ± 0.12^a	1.22 ± 0.08	0.93 ± 0.11^b	1.01 ± 0.05^b
TBARS – Kidney*	1.44 ± 0.06	2.12 ± 0.15^a	1.71 ± 0.13^c	1.55 ± 0.07	1.47 ± 0.06^b
HP – Liver*	64.35 ± 5.59	91.58 ± 2.59^a	76.13 ± 4.33	68.67 ± 5.79^b	70.08 ± 4.57^c
HP – Kidney*	51.67 ± 3.7	80.75 ± 5.59^a	65.78 ± 4.69	61.93 ± 2.52^c	62.48 ± 3.6^c
GSH – Liver**	43.53 ± 3.16	21.13 ± 1.52^a	33.9 ± 3.88^c	41.02 ± 2.7^b	40.83 ± 2.18^b
GSH – Kidney**	20.12 ± 1.92	4.12 ± 0.89^a	12.02 ± 1.08^b	17.83 ± 1.5^b	18.18 ± 2.08^b
SOD – Liver^#	6.78 ± 0.23	3.22 ± 0.3^a	5.57 ± 0.63^b	6.22 ± 0.28^b	6.48 ± 0.32^b
SOD – Kidney^#	14.25 ± 1.14	6.77 ± 0.88^a	10.15 ± 0.77^c	12.33 ± 1.02^b	13.23 ± 0.58^b
CAT – Liver^##	82.42 ± 3.44	46.28 ± 4.02^a	64.93 ± 5.85^c	73.08 ± 5.83^b	71.72 ± 3.49^b
CAT – Kidney^##	34.12 ± 2.34	19.2 ± 1.35^a	29.58 ± 1.73^b	32.02 ± 1.81^b	32.85 ± 1.19^b

* mmoles/100 g wet tissue.

** mg/100 g wet tissue.

^# U/mg of protein, one unit is defines as the enzyme concentration require to inhibit the OD at 560 nm of chromogen produced 50% in 1 min.

^## μmoles of H₂O₂ consumed/min/mg protein.

Values are expressed as mean ± SE (n = 6).

^ap < 0.01 compared with normal control group.

^bp < 0.01, ^cp < 0.05 compared with diabetic control group.