Figures & data
Figure 1. RT-PCR analysis of uPA and Ets-1 genes. After SKOV3 cells were cultured in basal medium and different concentrations of bFGF (0.1, 1, 10, 100 ng/mL) for 24 h, a significant change of expression of uPA and Ets-1 mRNA of SKOV3 cells was observed. The increase of both genes presented a dose-dependent response. β-actin was functioning here as a housekeeping gene.
![Figure 1. RT-PCR analysis of uPA and Ets-1 genes. After SKOV3 cells were cultured in basal medium and different concentrations of bFGF (0.1, 1, 10, 100 ng/mL) for 24 h, a significant change of expression of uPA and Ets-1 mRNA of SKOV3 cells was observed. The increase of both genes presented a dose-dependent response. β-actin was functioning here as a housekeeping gene.](/cms/asset/0fcf4990-7e48-4212-9f11-8cab0df19600/iphb_a_406436_f0001_b.gif)
Figure 2. Effect of bFGF on secretion of uPA protein. Each bar represents mean ± SEM. After treatment with different concentrations of bFGF (0.1, 1, 10, 100 ng/mL), supernantant was obtained from cultures of SKOV3 cells, and tested by ELISA. The expression of uPA protein increased due to stimulation with bFGF in a dose-dependent manner.
![Figure 2. Effect of bFGF on secretion of uPA protein. Each bar represents mean ± SEM. After treatment with different concentrations of bFGF (0.1, 1, 10, 100 ng/mL), supernantant was obtained from cultures of SKOV3 cells, and tested by ELISA. The expression of uPA protein increased due to stimulation with bFGF in a dose-dependent manner.](/cms/asset/8bc16c41-4839-4a1f-87c2-55c063896c79/iphb_a_406436_f0002_b.gif)
Table 1. Effect of bFGF on cell invasion of SKOV3 cells and the effect of antisense oligonucleotides on that procedure.