Figures & data
Figure 1. Mitogenic activity of damnacanthal on mouse thymocytes was determined by using MTT assay. Mouse thymocytes were isolated and incubated with increasing concentrations (0.468 μg/mL–30 μg/mL) of damnacanthal or Con A (2.5 μg/mL, as positive control) in culture medium for 24, 48, and 72h. Results were expressed as mean percentage ratio of MTT absorbance in damnacanthal-treated and control well ± standard error of three independent experiments with three wells each.
![Figure 1. Mitogenic activity of damnacanthal on mouse thymocytes was determined by using MTT assay. Mouse thymocytes were isolated and incubated with increasing concentrations (0.468 μg/mL–30 μg/mL) of damnacanthal or Con A (2.5 μg/mL, as positive control) in culture medium for 24, 48, and 72h. Results were expressed as mean percentage ratio of MTT absorbance in damnacanthal-treated and control well ± standard error of three independent experiments with three wells each.](/cms/asset/f897ca72-76af-4fdd-8e58-0ff30d09aeed/iphb_a_416976_f0001_b.gif)
Figure 2. Mitogenic activity of damnacanthal on PBMC was tested by using MTT assay. PBMC were isolated and incubated with increasing concentrations (0.468 μg/mL–30 μg/mL) of damnacanthal or PWM (50 μg/mL, as positive control) in culture medium for 24, 48, and 72h. Results were expressed as mean percentage ratio of MTT absorbance in damnacanthal-treated and control well ± standard error of three independent experiments with three wells each.
![Figure 2. Mitogenic activity of damnacanthal on PBMC was tested by using MTT assay. PBMC were isolated and incubated with increasing concentrations (0.468 μg/mL–30 μg/mL) of damnacanthal or PWM (50 μg/mL, as positive control) in culture medium for 24, 48, and 72h. Results were expressed as mean percentage ratio of MTT absorbance in damnacanthal-treated and control well ± standard error of three independent experiments with three wells each.](/cms/asset/224f73f7-05ec-4a67-b10a-6ad775f3442b/iphb_a_416976_f0002_b.gif)
Table 1. Flow cytometry analysis of cell cycle distribution on PBMC based on proliferation effect of damnacanthal and PWM. The values were the means ± SE of three experiments.
Figure 3. The production of human interleukin-2 in culture supernatants upon stimulation of PBMC by damnacanthal, and PWM. PBMC were isolated and incubated at 24, 48, and 72 h with active concentrations of damnacanthal at 30 μg/mL, while PWM at 50 μg/mL and interleukin-2 induction was specifically determined by ELISA.
![Figure 3. The production of human interleukin-2 in culture supernatants upon stimulation of PBMC by damnacanthal, and PWM. PBMC were isolated and incubated at 24, 48, and 72 h with active concentrations of damnacanthal at 30 μg/mL, while PWM at 50 μg/mL and interleukin-2 induction was specifically determined by ELISA.](/cms/asset/fc42650b-630c-4421-ae00-107117bb8c89/iphb_a_416976_f0003_b.gif)