Antitumor activity of polysaccharides isolated from Patrinia heterophylla

Figures & data

Figure 1.  Elution curve of polysaccharides of Patrinia heterophylla by DEAE- cellulose chromatography.

Table 1.  Content of carbohydrate, uronic acids and protein of PHB-P1 (%).

Sample	carbohydrate content	uronic acid content	protein content
PHB-P1	89.5 ± 4.12	23.5 ± 1.79	3.71 ± 0.46
PHB-P2	78.7 ± 3.43	19.8 ± 1.59	4.33 ± 0.34

Table 2.  Effect of PHB-P1 treatment on the tumor growth.

Group	Dose (mg/kgbw)	Tumor weight (g)	Inhibition ratio (%)
Control	Vehicle	2.46 ± 0.17
CTX	25	1.11 ± 0.19^a**	54.88
PHB-P1	30	1.62 ± 0.31^a*	34.15
	60	1.38 ± 0.29^a*	43.90
PHB-P2	30	2.40 ± 0.18^b**c*d*	2.44
	60	2.42 ± 0.25^b**c*d*	1.63

Values were expressed as mean ± SD. Statistical significance, ^**p <0.01 and ^* p <0.05.

^aAs compared with the control group.

^bAs compared with the CTX treatment group.

^cAs compared with the PHB-P1 (30 mg/kgbw) treatment group.

^dAs compared with the PHB-P1 (60 mg/kgbw) treatment group.

Figure 2.  Pathology sections of tumour tissues of the experimental mice (×200). (a) The control group; (b) The CTX treatment group; (c) PHB-P1 (30 mg/kg b.w.) treatment group; (d) PHB-P1 (60 mg/kg b.w.) treatment group.

Table 3.  Detection of apoptotic cells by TUNEL assay.

Group	Dose (mg/kgbw)	Apoptotic cells (%)
Control	Vehicle	4.05 ± 1.57
CTX	25	26.01 ± 2.24^**
PHB-P1	30	19.96 ± 2.03^**
	60	21.25 ± 1.91^**

Values were expressed as mean ± SD. Significant differences compared with the control group, ^**p <0.01.

Table 4.  Activity of serum LDH and AKP in U14-bearing mice.

Group	Dose (mg/kgbw)	LDH activity (U/L)	AKP activity (U/L)
Control	Vehicle	4216.21 ± 107.45	66.91 ± 11.03
CTX	25	4192.14 ± 119.84	68.32 ± 12.37
PHB-P1	30	3418.65 ± 131.53^**	70.15 ± 11.98
	60	3206.74 ± 152.37^**	72.67 ± 10.51

Values were expressed as mean ± SD. Significant differences compared with the control, ^**p <0.01.

Table 5.  Effect of PHB-P1 on distribution of cell cycle.

Group	Dose (mg/kgbw)	G0/G1	S	G2/M
Control	Vehicle	34.3 ± 1.01	38.6 ± 1.65	27.1 ± 1.17
CTX	25	37.9 ± 1.62	22.3 ± 0.96^**	39.9 ± 1.12^**
PHB-P1	30	29.7 ± 1.77	26.4 ± 1.54^**	43.9 ± 1.03^**
	60	28.1 ± 1.61	22.1 ± 1.33^**	49.8 ± 0.98^**

Values were expressed as mean ± SD. Significant differences compared with the control group, ^**p <0.01.

Table 6.  Effects of PHB-P1 on the expression of p53, Bax and Bcl-2 of tumor.

Group	Dose (mg/kgbw)	p53 positive cells (%)	Bax positive cells (%)	Bcl-2 positive cells (%)
Control	Vehicle	22.64 ± 5.87	19.52 ± 4.31	76.32 ± 8.25
CTX	25	58.67 ± 5.64^**	41.13 ± 7.56^**	50.19 ± 7.46^*
PHB-P1	30	57.43 ± 7.12^**	31.45 ± 5.02^*	52.01 ± 7.02^*
	60	63.55 ± 6.65^**	33.96 ± 6.12^*	48.71 ± 6.34^**

Results are expressed as mean ± SD. Significant differences compared with the control, ^**p <0.01 and ^*p <0.05.