Immunomodulatory activity of petroleum ether extract of Anacyclus pyrethrum

Figures & data

Table 1.  Effect of petroleum ether extract of Anacyclus pyrethrum on non-specific and specific immune function.

Group	Mean % edema (24 h)	% Change in DTH reaction (24 h)	Phagocytic index	% survival rate
Control	26.81 ± 1.39	_	1.0 ± 0.0	33.02 ± 8.75
CP	21.9 ± 0.57	18.22 ± 2.12*	0.47 ± 0.11	16.61 ± 5.92
PEE50	35.72 ± 1.03	33.24 ± 2.54 **	1.72 ± 0.34**	66.23 ± 9.12**
PEE100	43.91 ± 0.84**	63.81 ± 6.26**	1.81 ± 0.42**	83.1 ± 9.79**

All values are expressed as mean ± SEM, n = 6.

*P <0.05 considered significant as compare to control; **P <0.01 considered significant as compared to control.

Control, vehicle control, no treatment; CP, cyclophosphamide (30 mg/kgbw) i.p. daily; PEE50, petroleum ether extract (50 mg/kgbw) p.o. daily; PEE100, petroleum ether extract (100 mg/kgbw) p.o. daily.

Table 2.  Effect of petroleum ether extract of Anacyclus pyrethrum on neutrophil adhesion in rats.

Group	TLC (10^3 /mm^3)		% Neutrophil		Neutrophil index		% Neutrophil adhesion
	UTB	TB	UTB	TB	UTB	TB
Control	7.85 ± 0.04	7.29 ± 0.08	41.02 ± 0.27	37.31 ± 0.06	321.96 ± 30.52	272.26 ± 27.92	15.31 ± 1.57
CP	7.13 ± 0.1	6.9 ± 0.1	40.01 ± 0.36	37.02 ± 0.73	284.12 ± 32.14	255.37 ± 30.41	10.12 ± 1.86*
PEE50	7.91 ± 0.08	7.52 ± 0.05	46.0 ± 0.57	40.02 ± 1.22	363.42 ± 27.56	300.8 ± 23.42	17.23 ± 1.29
PEE100	8.12 ± 0.09	7.5 ± 0.09	47.6 ± 0.71	39.51 ± 0.44	385.51 ± 25.91	296.82 ± 21.56	23.14 ± 0.87**

All values are expressed as mean ± SEM, n = 6.

*P <0.05 considered significant compared to control; **P <0.01 considered significant compared to control.

Control, vehicle control, no treatment; PEE50, petroleum ether extract (50 mg/kgbw) p.o. daily; PEE100, petroleum ether extract (100 mg/kgbw) p.o. daily; CP, cyclophosphamide (30 mg/kgbw) i.p. daily.

Figure 1.  Effect of Anacyclus pyrethrum on cyclophosphamide induced suppression of hematological parameters.

Table 3.  Effect of petroleum ether extract of Anacyclus pyrethrum on HA titer value and IgG level after immunization in albino rats.

Groups	HA titer value	IgG level
		Primary anti body response	Secondary antibody response
Control	149.32 ± 26.91	0.7 ± 0.01	1.74 ± 0.01
CP	85.31 ± 13.41	0.54 ± 0.01	1.6 ± 0.01*
CP + PEE50	160.01 ± 12.62	0.74 ± 0.0	1.79 ± 0.01
CP + PEE100	245.32 ± 10.74*	0.8 ± 0.0	1.83 ± 0.0
PEE50	469.34 ± 52.1**	0.89 ± 0.01	2.02 ± 0.03**
PEE100	640.02 ± 78.16**	0.96 ± 0.01	2.12 ± 0.03**

All values are expressed as mean ± SEM, n = 6.

*P <0.05 considered significant as compare to control; **P <0.01 considered significant as compared to control.

Control, no drug; CP, cyclophosphamide (30 mg/kgbw) i.p. daily; CP + PEE50, cyclophosphamide (30 mg/kgbw) i.p. with petroleum ether extract (50/kgbw) p.o. daily; PEE50, petroleum ether extract (50 mg/kgbw) p.o. daily; CP + PEE100, cyclophosphamide (30 mg/kgbw) i.p. with petroleum ether extract (100 mg/kgbw) p.o. daily; PEE100, petroleum ether extract (100 mg/kgbw) p.o. daily.