Figures & data
Figure 1. Construction of DsbC-K2S fusion plasmid. (A) Identification of DsbC-K2S fusion plasmid by restriction digestion. Lane 1: 1-kb DNA marker. Lane 2: pET40b plasmid digested with BamHI and XhoI. Lane 3: DsbC-K2S plasmid digested with BamHI and XhoI. (B) Map of the DsbC- K2S fusion plasmid.
![Figure 1. Construction of DsbC-K2S fusion plasmid. (A) Identification of DsbC-K2S fusion plasmid by restriction digestion. Lane 1: 1-kb DNA marker. Lane 2: pET40b plasmid digested with BamHI and XhoI. Lane 3: DsbC-K2S plasmid digested with BamHI and XhoI. (B) Map of the DsbC- K2S fusion plasmid.](/cms/asset/bf900e7a-3a85-4a69-ad01-7ad78a4ccfe0/iphb_a_531482_f0001_b.gif)
Figure 2. The optimal expression conditions on K2S expression. Lane 1: protein marker. Lane 2: the expression product of pET40b (+)-K2S induced by optimal expression conditions. Lane 3: the expression product of pET40b (+) vector.
![Figure 2. The optimal expression conditions on K2S expression. Lane 1: protein marker. Lane 2: the expression product of pET40b (+)-K2S induced by optimal expression conditions. Lane 3: the expression product of pET40b (+) vector.](/cms/asset/b90fe286-edd3-436e-a7e5-dc4add2a6de6/iphb_a_531482_f0002_b.gif)
Figure 3. The elution curve of the Ni2+-chelating affinity chromatography. Fifty microliters of buffer NTA-0 was used to balance the column, then the sample was added. From 0 to 22.5 min was the penetration. After eluted with the elution buffer gradiently. The target protein was obtained at 46–73 min when the imidazole concentration reached at 10 mM.
![Figure 3. The elution curve of the Ni2+-chelating affinity chromatography. Fifty microliters of buffer NTA-0 was used to balance the column, then the sample was added. From 0 to 22.5 min was the penetration. After eluted with the elution buffer gradiently. The target protein was obtained at 46–73 min when the imidazole concentration reached at 10 mM.](/cms/asset/a62c0122-8501-49bc-a7ac-464feca98b64/iphb_a_531482_f0003_b.gif)
Figure 4. The SDS-PAGE of the purified DsbC-K2S fusion protein. Lane 1: protein marker; lane 2: the supernatant of the recombinant cell lysate; lane 3: penetration; lane 4: purified DsbC-K2S fusion protein.
![Figure 4. The SDS-PAGE of the purified DsbC-K2S fusion protein. Lane 1: protein marker; lane 2: the supernatant of the recombinant cell lysate; lane 3: penetration; lane 4: purified DsbC-K2S fusion protein.](/cms/asset/6d4e30a3-4e07-4cd0-bf62-ec7c0efcf9bf/iphb_a_531482_f0004_b.gif)