Antiproliferative and antioxidant activities of Juglans regia fruit extracts

Figures & data

Figure 1.  Dose-dependent graphs of antiproliferative activity of various fractions of Juglans regia extract (AKH-1 to AKH-7) against various human cancer cell lines.

Table 1.  Antiproliferative activity of extracts of Juglans regia fruits against various human cancer cell lines by MTT assay.^a

S. No.	Extract/fraction	Human cancer cell lines
		WRL, IC50 (µg/mL)	HEPG-2, IC50 (µg/mL)	KB, IC50 (µg/mL)	Caco2, IC50 (µg/mL)	MCF-7, IC50 (µg/mL)
1	Hexane fraction (AKH-1)	255 ± 22.5	301.6 ± 27.4	201.6 ± 12.5	301.6 ± 22.5	403 ± 35.1
2	Chloroform fraction (AKH-2)	60.6 ± 4.0	9.0 ± 1.0	40 ± 5.0	35.66 ± 6.0	>500
3	Ethyl acetate fraction (AKH-3)	70 ± 5.0	15.3 ± 2.5	50.3 ± 5.5	200 ± 10.0	448.3 ± 17.5
4	Methanol-soluble fraction (AKH-4)	455 ± 27.8	351.6 ± 22.5	351.6 ± 22.5	301 ± 22.5	350 ± 25.1
5	Methanol-insoluble fraction (AKH-5)	351 ± 32.5	298.3 ± 27.5	353 ± 15.2	356.6 ± 20.8	500 ± 30
6	Methanol extract (AKH-6)	405 ± 22.5	301 ± 22.5	403 ± 35.1	305 ± 5	348 ± 17.5
7	Aqueous methanol extract (AKH-7)	55 ± 10	66 ± 6.5	251.6 ± 22.6	>500	>500
8	Paclitaxel (Taxol)	0.004	n.d.	0.001	0.007	0.005
9	Podophyllotoxin	2.0	2.0	8.5	0.001	3.5
10	Tamoxifen	0.01	0.85	0.005	0.15	0.01

^aIC₅₀ values: < 20 µg/mL = potent activity, 20–200 µg/mL = good activity, 200–500 µg/mL = poorly active, and >500 µg/mL can considered as inactive. n = 3, three independent experiments in triplicate.

Figure 2.  Total phenolic content, antioxidant activity, and reducing power capacity of Juglans regia fruits extracts/fractions (at 200 µg) (results present the average and standard deviation of three independent measurements).