Formulation and comparative evaluation of poly herbal anti-acne face wash gels

Figures & data

Table 1. Ingredients used in the formulation.

Gel-HPMC		Gel-CRB
Ingredient	Quantity	Ingredient	Quantity
HPMC (hydroxy propyl methyl cellulose)	3.0 g	Carbopol 940	500 mg
Glycerin	50 ml	Glycerin	50 ml
Lauric acid	10 mg	Lauric acid	10 mg
Triethanolamine	1.0 ml	Triethanolamine	1.0 ml
Methyl paraben	0.5 mg	Methyl paraben	0.5 mg
Propyl paraben	0.5 mg	Propyl paraben	0.5 mg
Distilled water	20 ml	Distilled water	20 ml

Table 2. pH, viscosity and spreadability of formulations.

Formulation	pH*	Viscosity*(cps)	Spreadability* (g·cm/s)
Gel-CRB 100	6.46 ± 0.04	8266 ± 0.11	3.1 ± 0.31
Gel-HPMC 50	6.82 ± 0.49	8742 ± 0.28	3.4 ± 0.14
Gel-HPMC 100	7.22 ± 0.37	7548 ± 0.34	2.2 ± 0.50
Gel-HPMC 200	7.34 ± 0.12	5164 ± 0.45	1.9 ± 0.33

*Mean of triplicate readings.

Table 3.  Stability studies of formulations.

Formulation	pH			Viscosity (cps)			Synerisis
	4°C	25°C	40°C	4°C	25°C	40°C	4°C	25°C	40°C
Gel-CRB 100	6.45	6.46	6.46	8265	8266	8266	No	No	No
Gel-HPMC 50	6.82	6.82	6.82	8740	8742	8747	No	No	No
Gel-HPMC 100	7.22	7.22	7.22	7548	7548	7550	No	No	No
Gel-HPMC 200	7.34	7.34	7.36	5163	5164	5164	No	No	No

Table 4.  Anti-acne efficacy of formulations.

Formulation	Absorbance*
Gel-CRB 100^a	0.1438 ± 0.02
Gel-HPMC 50^a	0.166 ± 0.07
Gel-HPMC 100^a	0.1432 ± 0.01
Gel-HPMC 200^a	0.1285 ± 0.03
Standard^b	0.1271 ± 0.04
Control	0.759 ± 0.03

*Mean of triplicate readings.

^a10% extract in distilled water. ^bConcentration of clindamycin (100 mg/ml).

Figure 1. Anti-acne efficacy of formulations.