Development of a cell immobilization technique for the conversion of polysialogangliosides to monosialotetrahexosylganglioside

Figures & data

Figure 1.  A schematic diagram of continuous production process of monosialotetrahexosylganglioside (GM₁).

Figure 2.  Effect of silk fibroin concentration on sialidase yield and cell leakage of gel.

Figure 3.  Effect of initial cell concentration on sialidase yield and cell leakage of gel.

Figure 4.  Effect of temperature on monosialotetrahexosylganglioside (GM₁) conversion ratio. GM₁ was produced by 5 kg of cell-immobilized gel in tandem packed-bed reactor at pH 7.5 and various feed flow rates.

Figure 5.  Effect of feed flow rate on monosialotetrahexosylganglioside (GM₁) production. GM₁ was produced by 5 kg of cell-immobilized gel in tandem packed-bed reactor at 35°C, pH 7.5, and various feed flow rates.

Figure 6.  The continuous monosialotetrahexosylganglioside (GM₁) productive strength in packed-bed reactor at the optimized conditions.

Figure 7.  The continuous monosialotetrahexosylganglioside (GM₁) product conversion ratio in packed-bed reactor at the optimized conditions.