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Research Article

Inhibition of TNF-α/IFN-γ induced RANTES expression in HaCaT cell by naringin

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Pages 810-814 | Received 19 Oct 2010, Accepted 19 Dec 2010, Published online: 18 Apr 2011

Figures & data

Figure 1.  Naringin inhibited RANTES production by HaCaT cells stimulated with tumor necrosis factor alpha (TNF-α)/interferon gamma (IFN-γ) at 48 h (n = 3).

Figure 1.  Naringin inhibited RANTES production by HaCaT cells stimulated with tumor necrosis factor alpha (TNF-α)/interferon gamma (IFN-γ) at 48 h (n = 3).

Figure 2.  Naringin decreased the relative fold change of RANTES gene in HaCaT cells stimulated with tumor necrosis factor alpha (TNF-α)/interferon gamma (IFN-γ).

Figure 2.  Naringin decreased the relative fold change of RANTES gene in HaCaT cells stimulated with tumor necrosis factor alpha (TNF-α)/interferon gamma (IFN-γ).

Figure 3.  Naringin decreased the expression of nuclear factor kappa B (NF-κB) p65 in nuclear extracts of HaCaT cells in western blot. 1, Untreated HaCaT cells, 2, cells treated with 0.5 mmol/L naringin, 3, cells stimulated with tumor necrosis factor alpha (TNF-α)/interferon gamma (IFN-γ). #Compared with untreated cells, P < 0.02. *Compared with cells stimulated with TNF-α/IFN-γ, P < 0.01.

Figure 3.  Naringin decreased the expression of nuclear factor kappa B (NF-κB) p65 in nuclear extracts of HaCaT cells in western blot. 1, Untreated HaCaT cells, 2, cells treated with 0.5 mmol/L naringin, 3, cells stimulated with tumor necrosis factor alpha (TNF-α)/interferon gamma (IFN-γ). #Compared with untreated cells, P < 0.02. *Compared with cells stimulated with TNF-α/IFN-γ, P < 0.01.

Figure 4.  Nuclear factor kappa B (NF-κB) P65-positive expression in HaCaT cells (SP × 400). (A) NF-κB P65-positive expression in cytoplasm of normal cells, (B) NF-κB P65-positive expression in nuclei of some cells stimulated with tumor necrosis factor alpha (TNF-α)/interferon gamma (IFN-γ) for 0.5 h, and (C) NF-κB p65 expression in cytoplasm of cells stimulated with 0.5 mmol/L naringin for 16 h and TNF-α/IFN-γ for 0.5 h.

Figure 4.  Nuclear factor kappa B (NF-κB) P65-positive expression in HaCaT cells (SP × 400). (A) NF-κB P65-positive expression in cytoplasm of normal cells, (B) NF-κB P65-positive expression in nuclei of some cells stimulated with tumor necrosis factor alpha (TNF-α)/interferon gamma (IFN-γ) for 0.5 h, and (C) NF-κB p65 expression in cytoplasm of cells stimulated with 0.5 mmol/L naringin for 16 h and TNF-α/IFN-γ for 0.5 h.

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