Ameliorative effect of Aegle marmelos leaf extract on early stage alloxan-induced diabetic cardiomyopathy in rats

Figures & data

Figure 1.  HPTLC profile of the ethanol extract.

Table 1.   Effect of various pharmacological interventions on the biochemical parameters in the diabetic rats.

Group	TBARS (nmoles/mg protein)	GSH (nmoles/mg protein)	CAT (mols of H2O2 /min/mg protein)	SOD (IU/mg protein)	LDH (IU/L)	CK (IU/L)
Nondiabetic	12.17 ± 1.24	43.20 ± 6.38	1.27 ± 0.19	16.98 ± 1.48	288.4 ± 26.83	184.19 ± 18.58
Diabetic control	38.72 ± 4.08^a	12.21 ± 0.77^a	0.37 ± 0.09^a	4.17 ± 0.73^a	505.6 ± 18.67^a	582.97 ± 13.72^a
Diabetes + vehicle	38.19 ± 4.81^a	12.07 ± 0.84^a	0.29 ± 0.07^a	4.18 ± 0.78^a	509.57 ± 25.24^a	573.08 ± 25.10^a
Diabetes + tolbutamide	19.49 ± 0.27^b	20.24 ± 0.12^b	0.86 ± 0.29^b	10.31 ± 2.15^b	410 ± 22.15^b	287.07 ± 21.64^b
Diabetes + Aegle extract (100 mg/kg)	19.47 ± 2.70^b	25.68 ± 2.85^b	0.87 ± 0.10^b	10.27 ± 1.04^b	392.91 ± 17.93^b	436.0 ± 32.10^b
Diabetes + Aegle extract (200 mg/kg)	14.15 ± 2.15^b	34.80 ± 2.45^b	0.91 ± 0.12^b	13.74 ± 1.70^b	333.5 ± 19.95^b	273.17 ± 16.56^b
Diabetes + Aegle extract (400 mg/kg)	13.14 ± 1.70^b	35.12 ± 2.55^b	1.01 ± 0.05^b	14.02 ± 1.98^b	331.12 ± 17.77^b	284.05 ± 21.77^b

All values are expressed as mean ± SEM.

^ap < 0.05 vs. nondiabetic.

^bp < 0.05 vs. diabetic control.

Figure 2.  Fasting blood glucose and total cholesterol levels in the serum on 14th day. All values are expressed as mean ± SEM. a = p < 0.05 vs. nondiabetic, b = p < 0.05 vs. diabetic control.

Figure 3.  Normalized plot of different biochemical parameters in diabetic rats. a = p < 0.05 vs. nondiabetic, b = p < 0.05 vs. diabetic control.

Figure 4.  Photomicrograph of heart showing normal architecture with well-preserved cytoplasm and absence of inflammatory cells and edema at 10× (A) and 40× (B); diabetic control heart shows marked infiltration of inflammatory cells, edema and necrosis (light stained portion) as marked by the arrows at 10× (C) and 40× (D); AGE-treated heart the inflammatory cells are markedly decreased and partial recovery of the hearts is observed as marked by the arrows at 10× (F) and 40× (G); tolbutamide-treated heart shows decreased inflammatory cells and congestion as marked by the arrows at 10× (G) and 40× (H).