Terpenoids of methanol extract of Clerodendrum infortunatum exhibit anticancer activity against Ehrlich’s ascites carcinoma (EAC) in mice

Figures & data

Figure 1.  HPLC chromatogram of methanol extract of C. infortunatum performed in HPLC system consisting of Agilent 1200 series with quaternary pump, PDA detector and reverse phase Zorbax SB C-18 column (50 mm × 4.6 mm diameter; particle size 1.8 µm).

Figure 2.  Chemical structure of oleanolic acid (A) and clerodinin A (B).

Table 1.  Effect of methanol extract of C. infortunatum (MECI) on survival time on Ehrlich’s ascites carcinoma (EAC) bearing mice.

Group	Median survival time (d)	% ILS
Normal	–	–
EAC control	19.42 ± 0.91	–
MECI 100	23.44 ± 2.69	20.70
MECI 200	27.57 ± 2.57	41.96
5-FU	44.28 ± 1.11	128.01

Values are given in mean ± SD (n = 6).

5-FU, 5 fluorouracil; ILS, increase in life span.

Table 2.  Effects of MECI on tumor growth of EAC cells bearing mice in vivo.

Group	Tumor volume (cc)	Viable cell count (%)	Non-viable cell count (%)
EAC control	4.24 ± 0.67	77.86 ± 4.91	22.14 ± 4.91
MECI 100	3.30 ± 0.24^a	65.35 ± 2.56^b	34.65 ± 2.56^b
MECI 200	2.27 ± 0.32^b	43.30 ± 2.17^b	56.70 ± 2.17^b
5-FU	0.95 ± 0.36^b	19.55 ± 2.03^b	80.46 ± 2.03^b

Values are mean ± SD (n = 6).

MECI, methanol extract of C. infortunatum; 5-FU, 5 fluorouracil.

^bp < 0.001, ^ap < 0.05.

Table 3.  Effect of MECI on hematological parameters of EAC bearing mice.

Group	Hb (g %)	RBC (million/mm^3)	WBC (thousand/mm^3)
Normal	13.58 ± 0.80^c	5.34 ± 0.69^c	5.6 ± 0.22^c
EAC control	7.50 ± 0.77	2.72 ± 0.45	18.31 ± 1.003
MECI 100	8.19 ± 0.37^ns	2.91 ± 0.11^ns	15.04 ± 0.76^c
MECI 200	9.76 ± 0.54^c	3.56 ± 0.28^a	9.87 ± 0.48^c
5-FU	12.38 ± 0.70^c	5.16 ± 0.66^c	6.49 ± 0.36^c

Values are mean ± SD (n = 6). Results were compared with EAC control group.

MECI, methanol extract of C. infortunatum; 5-FU, 5 fluorouracil.

^cp < 0.001, ^bp < 0.01, ^ap < 0.05, ^nsnot significant.

Figure 3.  Effect of methanol extract of C. infortunatum on lipid peroxidation (A) glutathione content (B) and catalase activity (C) in EAC treated mice.