Chemical composition and antibacterial activity of a hexane extract of Tunisian caprifig latex from the unripe fruit of Ficus carica

Figures & data

Table 1.  Chemical composition of hexane extract obtained by GC-MS.

Retention time (min)	Area (%)	Constituent	Wiley library reference no.	CAS no.	Qual
4.70	0.06	Germacrene-D	121792	023986-74-5	98
5.04	0.01	Delta-Cadinene	121465	000483-76-1	97
7.03	0.33	2, 6, 10-Dodecatrien-1-ol	148283	004602-84-0	83
8.88	0.05	Dodecan-2-on	93122	006175-49-1	72
9.09	0.38	Hexadecanoic acid	213894	000112-39-0	98
10.73	0.33	Cis-Linoleic acid methyl ester	243137	000112-63-0	99
11.69	0.08	Pentadecane (CAS)	134011	000629-62-9	96
11.88	0.04	1, E-8, Z-10-Hexadecatriene	145418	080625-33-8	87
12.76	0.03	Tricosane (CAS)	275685	000638-67-5	96
13.90	0.13	Triacontane	34922	000638-68-6	91
14.51	0.02	8-Dimethoxynaphthalene	120716	105372-17-6	90
15.76	0.51	bis (2-Ethylhexyl) phthalate	326908	000117-81-7	91
16.30	0.05	Hexacosane	311168	000630-01-3	94
16.72	0.06	7-Pentadecyne	128099	022089-89-0	64
17.55	0.13	Heptacosane	32679	000593-49-7	95
18.79	0.10	Octacosane (CAS)	329269	000630-02-4	96
19.23	0.33	Squalene	337959	007683-64-9	93
20.05	0.13	Nanocosane (CAS)	337002	000630-03-5	97
20.61	0.37	Oxirane	345735	007200-26-2	89
21.73	0.29	1-Ethyl-3-acetyl-5	182541	112482-88-9	86
22.51	0.72	4-Methoxycarbonyl-2, 6-diphenylpyridine	237065	069209-39-8	74
22.98	1.04	Bornanone-3	49880	013854-85-8	70
23.38	0.19	1-Ethyl-3-acetyl-5	182541	112482-88-9	78
23.54	0.15	1, 6, 10, 14, 18, 22-Tetracosahexaen-3-ol	345737	054159-46-5	64
23.67	1.02	[3.2] Metacyclophane-10-ene	145464	121733-15-1	91
24.79	0.77	5-HYDROXY-6	236998	063955-63-5	78
27.96	0.98	9, 19-Cyclolanost-24-en-3-ol	345634	000469-38-5	93
28.96	0.94	beta-Amyrin	345611	000559-70-6	83
29.47	13.17	Lanosta-8	360770	002671-68-3	96
30.20	0.24	6-aza-B-Homo-5αcholestane	345497	066233-39-4	92
30.25	0.37	Lupeol	345599	000545-47-1	87
32.33	21.52	Urs-12-en-24-oic acid	360707	020475-86-9	91
32.75	15.63	Aristolone	141923	006831-17-0	86
32.95	23.47	Olean-12-en-3-ol, acetate	360750	001616-93-9	93
33.38	8.78	Maragenin I acetate	360711	071545-16-9	89
33.62	2.32	α-Amyrenyl acetate	360746	000863-76-3	64

Total terpene: 4.81%; Total coumarin: 90.56%; Qual, Quality.

Table 2.  Chemical formula and molecular weight of major component of Jrani hexane latex extract.

Name	CAS	Molecular formula	Molecular weight
Aristolone	006831-17-0		218.33908
Lanosta-8	002671-68-3		468.76368
Olean-12-en-3-ol, acetate	001616-93-9		468. 76368
Urs-12-en-24-oic acid	020475-86-9		468.36
alpha.-Amyrenyl acetate	000863-76-3		468.76368
Bornanone-3	013854-85-8		152.23644

Figure 1.  GS-MS chromatogram of analyzed hexane extract of Jrani caprifig latex.

Table 3.  Antimicrobial activity of the extract in Agar diffusion assay.

Strains	Growth inhibition zone diameter^a (mm)
	Hexane extract	Erythromycin (15 µg/disc)	Oxacillin (5 µg/disc)	Tetracycline (30 UI)	DMSO (10%)
Pseudomonas aeruginosa ATCC 27950	14	–	–	9	–
Pseudomonas aeruginosa ATCC 2783	14	–	–	14	–
Staphylococcus aureus ATCC 25923	28	22	28	22	–
Escherichia coli ATCC 25922	22	–	–	24	–
Enterococcus faecalis ATCC 29212	15	16	10	22	–
Staphylococcus epidermidis	26	–	–	–	–
Staphylococcus saprophyticus	28	21	27	32	–
Staphylococcus aureus	18	24	24	34	–
Enterobacter cloacae	19	–	–	21	–
Escherichia coli	20	–	–	16	–

–, no activity;

^aInhibition zones including diameter of the paper disc (6 mm).

Table 4.  Minimum inhibitory concentration (µg/mL) of the extract.

Strains	MIC (µg/mL)
	Hexane extract	Tetracycline
Pseudomonas aeruginosa ATCC 27950	312	100
Pseudomonas aeruginosa ATCC 2783	312	100
Staphylococcus aureus ATCC 25923	19	25
Escherichia coli ATCC 25922	39	10
Enterococcus faecalis ATCC 29212	312	25
Staphylococcus epidermidis	39	100
Staphylococcus saprophyticus	19	1
Staphylococcus aureus	78	1
Enterobacter cloacae	78	25
Escherichia coli	78	10