Antithrombotic effect of a novel protein from Fusarium sp. CPCC 480097 in a rat model of artery-vein bypass thrombosis

Figures & data

Table 1.  Effects of Fu-P on artery-vein bypass thrombosis (n = 10).

Group	Dose (mg/kg)	Thrombus mass (mg)	Inhibition ratio (%)
Saline	2	52.19 ± 9.31	–
Heparin	0.01	43.84 ± 10.48*	16.00
	0.6	29.99 ± 2.38**	42.53
	0.01	41.55 ± 8.2*	20.39
Fu-P	0.03	32.44 ± 4.81**	37.84
	0.1	21.71 ± 7.74**	58.40

* p < 0.05 compared with negative control.

** p < 0.01 compared with negative control.

Table 2.  Effects of Fu-P on FCT.

Group	FCT (s)
Saline	10.2 ± 2.1
A	ND
B	50.2 ± 2.7, then the clot disappears at 150 ± 3.2 s
C	ND
D	126.5 ± 2.8

The results shown represent averages from three independent experiments. Error bars represent the standard deviation.

FCT, fibrinogen clotting time.

Table 3.  Effects of Fu-P on rat plasma coagulant time (n = 10).

Group	Dose (mg/kg)	PT (s)	APTT (s)	TT (s)
Saline	2	9.2 ± 0.6	17.7 ± 1.7	45.1 ± 4.0
Heparin	0.01	9.4 ± 0.4	18.7 ± 1.0	47.9 ± 3.3
	0.6	11.2 ± 0.4	134.5 ± 22.2**	92.4 ± 11.6**
	0.01	9.4 ± 0.4	21.2 ± 2.2	49.7 ± 5.2
Fu-P	0.03	9.5 ± 0.4	25.7 ± 5.5	55.1 ± 6.6*
	0.1	13.0 ± 2.7**	151.5 ± 14.2**	103.3 ± 8.7**

* p < 0.05 compared with negative control.

** p < 0.01 compared with negative control.

APTT, activated partial thrombinplastin time; PT, prothrombin time; TT, thrombin time.

Figure 1.  The effects of Fu-P dose on the concentration of FBG. The results shown represent averages from three independent experiments. Error bars represent the standard deviation.

Figure 2.  Effects of Fu-P on Xa activity using S-2765 as the substrate (•) and thrombin activity using S-2238 as the substrate (▪). The results shown represent averages from three independent experiments. Error bars represent the standard deviation.