Figures & data
Figure 1. Chemical equilibrium reaction between the lactone (a) and carboxylate (b) forms of CPTs (R=H, CPT; R=OH, HCPT).
![Figure 1. Chemical equilibrium reaction between the lactone (a) and carboxylate (b) forms of CPTs (R=H, CPT; R=OH, HCPT).](/cms/asset/12f9f7e4-461f-4ac9-aedd-208455ecf688/iphb_a_654228_f0001_b.gif)
Figure 2. Representative chromatograms of: (A) a blank mouse liver tissue homogenate sample without the first LLE step; (B) a blank mouse liver tissue homogenate sample using the two-step LLE; (C) a blank mouse liver tissue homogenate spiked with HCPT (20 ng/mL) and I.S. (CPT 200 ng/mL); (D) a mouse liver tissue homogenate sample at 4 h after oral administration of HCPT at a dose of 80 mg/kg and spiked with I.S. (CPT 200 ng/mL).
![Figure 2. Representative chromatograms of: (A) a blank mouse liver tissue homogenate sample without the first LLE step; (B) a blank mouse liver tissue homogenate sample using the two-step LLE; (C) a blank mouse liver tissue homogenate spiked with HCPT (20 ng/mL) and I.S. (CPT 200 ng/mL); (D) a mouse liver tissue homogenate sample at 4 h after oral administration of HCPT at a dose of 80 mg/kg and spiked with I.S. (CPT 200 ng/mL).](/cms/asset/7887058d-a18e-4359-9ea3-d7fe8e636ab4/iphb_a_654228_f0002_b.gif)
Table 1. Accuracy and precision for the analysis of HCPT in mouse liver tissue homogenates.
Table 2. Stability of HCPT in liver tissue homogenate samples (n = 6).
Table 3. The pharmacokinetic parameters of HCPT after oral administration of 80 mg/kg of HCPT.