Pearl extract enhances the migratory ability of fibroblasts in a wound healing model

Figures & data

Figure 1.  Optical photomicrographs of Hs68 cells cultured on TCPS in medium in the absence or presence of PL after 7 days of incubation. Scale bar = 100 µm.

Figure 2.  Metabolic activity of Hs68 cells cultured in the absence or presence of PL on TCPS after 7 days of incubation was assayed by MTT. Data were determined from three independent cultures and expressed as mean ± standard deviation.

Table 1.  Average numbers of migratory Hs68 cells were counted in the scratch site at 0 and 16 h of incubation.

Medium	Hs68 cell
	Total number of cell in scratch site		Number of miratory cell
	0 h	16 h	16–0 h
PL−	3 ± 1	11 ± 2	8 ± 2
PL+	3 ± 1	29 ± 1	26 ± 2

Data was determined from three independent cultures and expressed as mean ± standard deviation.

Figure 3.  Dynamical process of in vitro scratch assay with Hs68 cells in medium in the (a) absence or (b) presence of PL on the scratched well at 16 h of incubation. Scale bar = 100 µm.

Figure 4.  The mRNA expression of Collagen Type I and Collagen Type III in Hs68 cells cultured in the absence or presence of PL on TCPS after 1 day of incubation. The results were presented as the ratio of gene expression of Collagen Type III relative to Collagen Type I. *Significant difference as determined by one-way ANOVA followed by the Duncan’s test (p < 0.05).

Table 2.  Average numbers of migratory HFFs were counted in the scratch site at 0 and 16 h of incubation.

Medium	HFFs
	Total number of cells in scratch site		Number of miratory cell
	0 h	16 h	0–16 h
PL−	2 ± 1	9 ± 1	7 ± 2
PL+	2 ± 2	22 ± 2	20 ± 3

Data were determined from three independent cultures and expressed as mean ± standard deviation.

Figure 5.  Dynamic process of the in vitro scratch assay with HFFs in medium in the (a) absence or (b) presence of PL in scratched wells after 16 h of incubation. Scale bar = 100 µm.