Antidiabetic components of Cassia alata leaves: Identification through α-glucosidase inhibition studies

Figures & data

Table 1.  Anti-α-glucosidase activity of C. auriculata extract, fractions and isolated compounds.

Sample	IC50 values*
	µg/ml	µM
Crude extract	63.75 ± 12.81	-
Petroleum ether	NA	-
Chloroform	44.25 ± 10.23**	-
Ethyl acetate	2.95 ± 0.47^†	-
n-Butanol	25.80 ± 2.01^†	-
Water	NA	-
Kaempferol	16.2 ± 2.2	56.7 ± 7.7
Kaempferol 3-O-gentiobioside	82.5 ± 13.7	50.0 ± 8.5
Acarbose	107.31 ± 12.31	166.21 ± 19.06

*Data are mean and SEM values obtained from 4-6 separate experiments. **Not significantly different from the crude extract treatment (P > 0.05). ^†Significantly different from the chloroform or crude extract treatment (P < 0.05).

NA, no activity up to the concentration of 200 µg/ml.

Figure 1.  Anti-α-glucosidase activity of the crude ethanol extract of C. alata leaves and its fractions. Data from a representative result shows mean and SEM values (n = 4).

Figure 2.  HPLC chromatograms of active fractions obtained from the crude ethanol extract of C. alata leaves. Note that one major component of the ethyl acetate (panel A) and n-butanol (panel B) fraction.

Figure 3.  Structures of major components of the active fractions: kaempferol from the ethyl acetate and kaempferol 3-O-gentiobioside from the n-butanol fraction.