Comparison of protective and curative potential of Daucus carota root extract on renal ischemia reperfusion injury in rats

Figures & data

Table 1. Data showing comparison of serum creatinine, uric acid, urea and blood urea nitrogen (BUN) in sham control (SC), disease control (DC) and treated group of petroleum ether extract (PEE 250 and 500 mg/kg), fractional methanol extract (FME 250 and 500 mg/kg) and direct methanol extract (DME 250 and 500 mg/kg) of D. carota root.

Groups	Creatinine (mg/dl)	Uric acid (mg/dl)	Urea (mg/dl)	BUN (mg/dl)
SC	1.030 ± 0.03786	1.150 ± 0.02887	53.31 ± 1.300	24.91 ± 0.6061
DC	3.090 ± 0.03786###	3.500 ± 0.1155###	132.0 ± 2.874###	61.68 ± 2.278###
PEE-250	1.217 ± 0.04410***	2.400 ± 0.05774***	91.92 ± 0.736***	42.95 ± 0.3459***
PEE-500	0.8533 ± 0.04910***	1.300 ± 0.05774***	58.26 ± 2.495***	27.23 ± 1.164***
FME-250	1.527 ± 0.07219***	2.633 ± 0.03333**	100.6 ± 1.334***	47.00 ± 0.6237***
FME-500	0.9600 ± 0.03606***	1.700 ± 0.1155***	77.17 ± 1.351***	36.06 ± 0.6319***
DME-250	1.400 ± 0.05774***	2.700 ± 0.05774*	107.9 ± 1.745*	50.42 ± 0.8146*
DME-500	1.173 ± 0.01453***	2.267 ± 0.08819***	84.75 ± 3.868***	39.60 ± 1.808***

Values are expressed in mean ± SEM, for each group (n = 6).

###p < 0.001 versus sham control, ***p < 0.001 versus disease control, *p < 0.05 versus disease control.

Table 2. Data showing comparison of SOD, catalase, MDA and glutathione in sham control (SC), disease control (DC) and treated group of petroleum ether extract (PEE 250 and 500 mg/kg), fractional methanol extract (FME 250 and 500 mg/kg) and direct methanol extract (DME 250 and 500 mg/kg) of D. carota root.

Groups	SOD (U/min/mg protein)	Catalase (mmol/min/mg protein)	MDA (nmol/mg protein)	Glutathione (mmol/mg protein)
SC	6.633 ± 0.03712	0.01711 ± 0.000206	28.55 ± 1.019	28.55 ± 0.7102
DC	2.88 ± 0.1852###	0.00445 ± 0.00416###	61.45 ± 1.623###	15.67 ± 1.07###
PEE-250	3.787 ± 0.1453**	0.00899 ± 0.000435***	48.46 ± 0.9597***	21.44 ± 0.4726***
PEE-500	5.34 ± 0.09539***	0.01455 ± 0.000598***	30.76 ± 1.054***	25.93 ± 0.8483***
FME-250	3.611 ± 0.1795*	0.00786 ± 0.000228***	53.11 ± 2.145*	20.11 ± 0.3522*
FME-500	4.35 ± 0.1345***	0.01191 ± 0.000362***	43.84 ± 1.002***	23.28 ± 0.7688***
DME-250	3.453 ± 0.1065*	0.00815 ± 0.000532***	51.69 ± 1.678**	20.56 ± 0.3437**
DME-500	4.225 ± 0.1117***	0.009267 ± 0.000546***	38.89 ± 0.9042***	23.32 ± 0.4323***

Values are expressed in mean ± SEM, for each group (n = 6).

###p < 0.001 versus sham control, ***p < 0.001 versus disease control, **p < 0.01 versus disease control, *p < 0.05 versus disease control.

Figure 1. Histopathology of kidney in protective studies.

Table 3. Data showing comparison of serum creatinine, uric acid, urea and BUN in sham control (SC), disease control (DC) and treated group of petroleum ether extract (PEE 250 and 500 mg/kg), fractional methanol extract (FME 250 and 500 mg/kg) and direct methanol extract (DME 250 and 500 mg/kg) of D. carota root in curative study.

Groups	Creatinine (mg/dl)	Uric acid (mg/dl)	Urea (mg/dl)	BUN (mg/dl)
SC	0.9675 ± 0.05023	1.4 ± 0.05774	31.27 ± 0.5816	17.89 ± 0.4561
DC	3.175 ± 0.125###	2.9 ± 0.1732###	66.51 ± 1.034###	31.08 ± 0.4919###
PEE-250	1.18 ± 0.02944***	1.847 ± 0.07219***	45.43 ± 0.8541***	21.93 ± 0.3754***
PEE-500	0.835 ± 0.05809***	1.2 ± 0.05774***	32.92 ± 0.849***	15.37 ± 0.3844***
FME-250	1.555 ± 0.107***	1.867 ± 0.08819***	53.07 ± 1.128***	24.83 ± 0.5364***
FME-500	1.038 ± 0.05865***	1.6 ± 0.05774***	42.33 ± 0.620***	22.43 ± 1.091***
DME-250	1.25 ± 0.06455***	2.333 ± 0.08819*	46.88 ± 1.132***	21.9 ± 0.5132***
DME-500	0.9225 ± 0.04697***	1.433 ± 0.1202***	36.06 ± 2.827***	16.83 ± 1.335***

Values are expressed in mean ± SEM, for each group (n = 6).

###p < 0.001 versus sham control, ***p < 0.001 versus disease control, *p < 0.05 versus disease control.

Table 4. Data showing comparison of SOD, catalase, MDA and glutathione in sham control (SC), disease control (DC), and treated group of petroleum ether extract (PEE 250 and 500 mg/kg), fractional methanol extract (FME 250 and 500 mg/kg), direct methanol extract (DME 250 and 500 mg/kg) of D. carota root in curative study.

Groups	SOD (U/min/mg protein)	Catalase (mmol/min/mg protein)	MDA (nmol/mg protein)	Glutathione (mmol/mg protein)
SC	7.41 ± 0.1514	0.01562 ± 0.000192	28.65 ± 0.6128	32.77 ± 1.066
DC	3.35 ± 0.05774###	0.00315 ± 0.000293###	61 ± 1.058###	15.96 ± 1.073###
PEE-250	4.3 ± 0.1893**	0.006777 ± 0.00013***	48.37 ± 0.2437***	21.64 ± 0.3531**
PEE-500	6.607 ± 0.1727***	0.01365 ± 0.000199***	29.71 ± 1.004***	29.78 ± 1.492***
FME-250	4.17 ± 0.1474*	0.00553 ± 0.000242***	54.7 ± 1.374**	19.29 ± 0.9015*
FME-500	4.593 ± 0.1244***	0.008503 ± 0.00028***	44.9 ± 0.993***	22.68 ± 0.5164***
DME-250	4.097 ± 0.06386*	0.0065 ± 0.000261***	50.41 ± 1.317***	20.7 ± 0.7305*
DME-500	5.35 ± 0.1185***	0.01048 ± 0.00029***	35.9 ± 0.4068***	27.56 ± 0.5138***

Values are expressed in mean ± SEM, for each group (n = 6).

###p < 0.001 versus sham control, ***p < 0.001 versus disease control, **p < 0.01 versus disease control, *p < 0.05 versus disease control.

Figure 2. Histopathology of kidney in curative studies.