Figures & data
Figure 1. HPLC–MS profile of the polyphenols obtained from ethanol extract of Haplophyllum tuberculatum aerial parts at 254 nm, 280 nm and 340 nm.
![Figure 1. HPLC–MS profile of the polyphenols obtained from ethanol extract of Haplophyllum tuberculatum aerial parts at 254 nm, 280 nm and 340 nm.](/cms/asset/b329135e-36c4-4318-afdf-94e122570800/iphb_a_819517_f0001_b.jpg)
Figure 2. Effect of Haplophyllum tuberculatum ethanol extract on cell viability. U373-MG cells were treated with plant extract (range of concentrations from 0.025 to 250 µg/mL) for 24 h. Triton X-100 was employed as negative control. Results were expressed as mean of the percentage of control cells (100%) ± standard deviation (S.D.). *p < 0.05 versus control cells; $p < 0.05 versus ethanol extracts at all assayed concentrations.
![Figure 2. Effect of Haplophyllum tuberculatum ethanol extract on cell viability. U373-MG cells were treated with plant extract (range of concentrations from 0.025 to 250 µg/mL) for 24 h. Triton X-100 was employed as negative control. Results were expressed as mean of the percentage of control cells (100%) ± standard deviation (S.D.). *p < 0.05 versus control cells; $p < 0.05 versus ethanol extracts at all assayed concentrations.](/cms/asset/9a24acd0-c40f-4ed3-92b2-e7fc79ea04db/iphb_a_819517_f0002_b.jpg)
Figure 3. Protective effect of Haplophyllum tuberculatum ethanol extract against H2O2-induced loss in viability. U373-MG cells were treated with plant extract (range of concentrations from 0.025 to 250 µg/mL) for 24 h, prior to 1 mM H2O2 exposure (30 min). Triton X-100 was employed as negative control. Results were expressed as a mean of the percentage of control cells (100%) ± standard deviation (S.D.). *p < 0.05 versus control cells; #p < 0.05 versus H2O2.
![Figure 3. Protective effect of Haplophyllum tuberculatum ethanol extract against H2O2-induced loss in viability. U373-MG cells were treated with plant extract (range of concentrations from 0.025 to 250 µg/mL) for 24 h, prior to 1 mM H2O2 exposure (30 min). Triton X-100 was employed as negative control. Results were expressed as a mean of the percentage of control cells (100%) ± standard deviation (S.D.). *p < 0.05 versus control cells; #p < 0.05 versus H2O2.](/cms/asset/b16ec129-2831-42e1-9396-a3e7e24e83be/iphb_a_819517_f0003_b.jpg)
Figure 4. Protective effect of Haplophyllum tuberculatum ethanol extract against H2O2-induced intracellular ROS production. U373-MG cells were treated with plant extract (0.025, 0.25 and 0.025 µg/mL) for 24 h, prior to 1 mM H2O2 exposure (30 min). Results were expressed as a mean of the percentage of control cells (100%) ± standard deviation (S.D.). *p < 0.05 versus H2O2.
![Figure 4. Protective effect of Haplophyllum tuberculatum ethanol extract against H2O2-induced intracellular ROS production. U373-MG cells were treated with plant extract (0.025, 0.25 and 0.025 µg/mL) for 24 h, prior to 1 mM H2O2 exposure (30 min). Results were expressed as a mean of the percentage of control cells (100%) ± standard deviation (S.D.). *p < 0.05 versus H2O2.](/cms/asset/d3a725ae-bd7c-4962-a0e0-6c028b15bb14/iphb_a_819517_f0004_b.jpg)