Antidiabetic effects of Mukia maderaspatana and its phenolics: An in vitro study on gluconeogenesis and glucose uptake in rat tissues

Figures & data

Figure 1. HPLC analysis of the chloroform extract of Mukia showing the presence of phloroglucinol and quercetin.

Figure 2. Gluconeogenesis from pyruvate in rat liver. In vitro gluconeogenesis in rat liver slices was tested in the absence and presence of pyruvate (5 mM) and in the absence and presence of 1 and 2 Units of insulin. Results are mg of glucose formed/g of liver.

Figure 3. Inhibition of gluconeogenesis in rat liver by quercetin and phloroglucinol in the absence and presence of 1 Unit of insulin. Gluconeogenesis in rat liver was determined from pyruvate in the presence of 0.25 mg/ml and 0.5 mg/ml of quercetin or phloroglucinol preincubated with liver slices in the absence or presence of 1 Unit of insulin prior to determining gluconeogenesis from pyruvate (5 mM).

Figure 4. Inhibition of gluconeogenesis in rat liver by Mukia extract in the absence and presence of 1 Unit of insulin. Gluconeogenesis in rat liver was determined from pyruvate in the presence of 0.25 mg/ml and 0.5 mg/ml Mukia extract in the absence or presence of 1 U insulin preincubated with liver slices prior to determining gluconeogenesis from pyruvate (5 mM).

Table 1. In vitro glucose uptake by isolated rat hemi-diaphragm.

Group	Glucose uptake (mg/dl/g/30 min)
Normal control	101.29 ± 9.24*
Insulin control	112.41 ± 9.14*
Mukia 50 µg/ml	101.49 ± 8.29
Mukia 100 µg/ml	99.16 ± 12.72
Insulin + Mukia 100 µg/ml	152.82 ± 13.30*

Glucose uptake in the isolated rat hemi-diaphragm was carried out in vitro as described in the Methods section. Values are expressed as mean ± SD (n = 8). *Results are significantly different among groups at p < 0.05.