Antimicrobial activity and chemical composition of fixed oil extracted from the body fat of the snake Spilotes pullatus

Figures & data

Table 1. Origins of the bacterial strains and their antibiotic resistance profiles.

Bacteria	Origin	Resistance profiles
Escherichia coli 27	Surgical wound	Ast, Ami, Amox, Ca, Cfc, Cf, Caz, Cip, Clo, Imi, Can, Szt, Tet, Tob
Staphylococcus aureus 358	Surgical wound	Oxa, Gen, Tob, Ami, Can, Neo, Para, But, Sis, Net
Pseudomonas aeruginosa 03	Uroculture	Cpm, Ctz, Imi, Cip, Ptz, Lev, Mer, Ami

Ast, aztreonam; Ami, amicillin; Amox, amoxicillin, Ca, cefadroxil; Cfc, cefaclor; Cf, cefalotin; Caz, ceftazidime; Cip, ciprofloxacin; Clo, chloramphenicol; Imi, imipenem; Can, kanamycin; Szt, sulfatrim; Tet, tetracycline; Tob, tobramycin; Oxa, oxacillin; Gen, gentamicin; Neo, neomycin; Para, paromomycin; But, butirosin; Sis, sisomicin; Net, netilmicin; Cpm, cfepime; Ctz, ceftazidime; Ptz, piperacillin-tazobactam; Lev, levofloxacin; Mer, meropene.

Table 2. Methyl esters of the fatty acids identified in the fixed oil extracted from the body fat of Spilotes pullatus by gas chromatography coupled to a mass spectrometer.

Fatty acid equivalent	RT (min)	(%)	Constituents
Saturated
Myristic acid	19.48	1.28	Methyl myristate
Palmitic acid	22.97	19.01^a	Methyl palmitate
Stearic acid	25.37	10.58^a	Methyl stearate
Nonadecanoic acid	27.07	2.72	Methyl nonadecanoate
Unsaturated
Palmitoleic acid	22.64	4.02	Methyl palmitoleate
Linoleic acid	25.09	17.28^a	Methyl linoleate
Elaidic acid	25.12	37.26^a	Methyl elaidate
Arachidonic acid	26.68	1.48	Methyl arachidonate
Eicosanoic acid	26.85	0.88	Methyl eicosa-7,10,13- trienoate
Docosahexaenoic acid	28.40	0.46	Methyl docosahexaenoate
Total saturated		33.59
Total unsaturated		61.38
General total		94.97

^aPrincipal fatty acids.

Table 3. Results of the trials using FOSP as a modulator of the actions of micro-diluted antifungal agents and controls (without FOSP); all results in µg/mL.

	C.T. 13803		C.A. 40046		C.K. 6258
Antifungal agents	FOSP	Control	FOSP	Control	FOSP	Control
Benzoilmetronidazol	≥1024	≥1024	≥1024	≥1024	≥1024	≥1024
Anfotericina B	≥1024	≥1024	≥1024	≥1024	≥1024	≥1024
Mebendazol	≥1024	≥1024	≥1024	≥1024	≥1024	≥1024
Nistatina	≥1024	≥1024	≥1024	≥1024	≥1024	≥1024

CT, Candida tropicalis; CK, Candida krusei; CA, Candida albicans.

Table 4. CIM values (µg/mL) of aminoglycosides in the absence and presence of the fixed oil from S. pullatus, against Staphylococcus aureus 358 (SA 358), Escherichia coli 27 (EC 27), and Pseudomonas aeruginosa 03 (PA 03).

Antibiotics	SA 358		EC 27		PA 03
	Control	FOSP	Control	FOSP	Control	FOSP
Amikacin	39.06	39.06	9.76	78.125 ^a	156.25	78.125
Gentamicin	39.06	2.44 ^a	39.06	9.76 ^a	39.06	9.76 ^a
Neomycin	9.76	19.53	39.06	156.25 ^a	625	312.5

^aSignificant results.