Effective antidiabetic and antioxidant fractions of Otostegia persica extract and their constituents

Figures & data

Figure 1. Structure of pure compounds; chrysoeriol 1, 6-methylapigenin 2, apigenin7-O-glucoside 3, echinaticin 4.

Figure 2. Comparison of antioxidant activity of different fractions and pure compounds of O. persica (1 mg/ml) with BHT (200 µg/ml) and vitamin E (50 µg/ml).

Table 1. Radical scavenging activity of different fractions and pure compounds of O. persica.

Fractions	Dose (mg/ml)	Inhibition % at 30 min
PE	0.10	15.51
	0.50	05.73
	1.00	10.61
CH	0.10	09.70
	0.50	19.40
	1.00	24.75
EA	0.10	18.18
	0.50	59.21
	1.00	81.96
BU	0.10	20.50
	0.50	54.50
	1.00	81.50
ME	0.10	10.16
	0.50	57.59
	1.00	91.53*
Chrysoeriol	1.00	76.93*
Apigenin glucoside	1.00	28.68
Echinaticin	1.00	40.82
BHT	0.20	90.60
Vitamin E	0.05	94.69

*Results comparable with BHT and vitamin E with t-test (p > 0.05).

Table 2. Antidiabetic activity of AI extract and different fractions of O. persica extract.

Groups	Dose (mg/kg)	Number of alive mice	Blood sugar reduction %
Control (NS)^a	–	5	05.96 ± 02.01
Control (NS/tween)^b	–	5	03.52 ± 01.04
ME	100	4	18.85 ± 04.71
ME	200	6	38.65 ± 06.80
ME	300	6	41.33 ± 07.64*
ME	400	6	67.32 ± 08.48*
AI	300	4	34.07 ± 08.55
AI	400	4	23.27 ± 06.15
PE^c	400	4	12.28 ± 05.57
CH^c	400	4	06.01 ± 03.14
EA	400	5	26.18 ± 05.89
BU	400	5	21.34 ± 06.27
Glibenclamide^d	3	4	62.38 ± 08.28
Insulin^d	12 IU	4	88.62 ± 04.12

Results demonstrated as mean ± SD.

*Comparable with positive controls with p > 0.05.

^aNegative control (normal saline).

^bNegative control (normal saline + tween 80).

^cFractions dissolved in normal saline + tween 80.

^dPositive controls.