In vitro enhancement of psoralen as an important anticancer compound in Psoralea corylifolia through precursor feeding

Figures & data

Table 1. Psoralen estimates obtained from callus derived from different parts P. corylifolia.

	Callus derived from different plant parts
Plant parts	Area (mAU)^a	Psoralen quantity (µg/g fresh wt.)
Cotyledon callus	139 008	2601.8
Node callus	100 262	1876.6
Leaf callus	78 429	1468.0
Root callus	56 729	1062.0

^aMilliabsorbance units.

Table 2. Effect of some precursors on psoralen accumulation (µg/g fresh wt.) in cotyledonary callus of P. corylifolia, after 30d of incubation.

	Cotyledonary callus
	Area (mAU)^a	Psoralen quantity (µg/g fresh wt.)
Control
0	83 193.0	1932.8
Umbelliferone (mg/l)
0.1	107 494.1	2011.9
1	108 910.0	2038.4
2.5	120 175.0	2249.3
5	123 138.0	2304.8
25	126 718.0	2371.7
50	125 731.0	2353.3
Cinnamic acid (mg/l)
0.1	130 108.0	2435.2
1	130 782.0	2447.8
2.5	134 577.0	2518.8
5	133 519.0	2499.4
25	121 398.0	2272.2
50	112 563.0	2106.8
NADPH (mg/l)
0.1	109 059.0	2041.2
1	113 921.6	2132.2
2.5	126 814.2	2373.7
5	124 153.0	2333.7
25	123 789.0	2315.4

^aMilliabsorbance units.