The effect of some antibiotics on glutathione reductase enzyme purified from liver and erythrocyte of Lake Van pearl mullet

Figures & data

Figure 1. SDS-polyacrylamide gel electrophoresis of GR purified by affinity chromatography.

Figure 2. Purification of liver homogenate GR by affinity chromatography and the columns (1.3 × 60 cm²) were eluted by buffer C at pH 7.3. It was buffer at 20 ml/h flow rate for fraction volumes of 6 ml.

Figure 3. Purification of erythrocyte hemolysate GR by affinity chromatography and the columns (1.3 × 60 cm²) were eluted by buffer C at pH 7.3. It was buffer at 20 ml/h flow rate for fraction volumes of 6 ml.

Figure 4. Optimal pH for liver and erythrocyte GR.

Figure 5. Optimal temperature for liver and erythrocyte GR.

Figure 6. Activity–ionic strength for liver and erythrocyte GR.

Table 1. K_i and IC₅₀ values obtained from regression analysis graphs for glutathione reductase in the presence of different drugs concentration.

Tissue	Antibiotics	IC50 (mM)	Ki (mM)	Inhibition type
Liver	Amikacin	16.3	13.9	Non-competitive
	Cefazolin	36.6	18.4	Non-competitive
	Ivermectin	0.504	0.654	Non-competitive
	Kanamycin	18.8	11.2	Non-competitive
Erythrocyte	Amikacin	20.0	23.2	Non-competitive
	Cefazolin	30.4	46.4	Non-competitive
	Ivermectin	0.787	1.19	Non-competitive
	Kanamycin	31.8	36.4	Non-competitive