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Original Article

The effect of some antibiotics on glutathione reductase enzyme purified from liver and erythrocyte of Lake Van pearl mullet

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Pages 1647-1652 | Received 08 May 2014, Accepted 01 Dec 2014, Published online: 05 May 2015

Figures & data

Figure 1. SDS-polyacrylamide gel electrophoresis of GR purified by affinity chromatography.

Figure 1. SDS-polyacrylamide gel electrophoresis of GR purified by affinity chromatography.

Figure 2. Purification of liver homogenate GR by affinity chromatography and the columns (1.3 × 60 cm2) were eluted by buffer C at pH 7.3. It was buffer at 20 ml/h flow rate for fraction volumes of 6 ml.

Figure 2. Purification of liver homogenate GR by affinity chromatography and the columns (1.3 × 60 cm2) were eluted by buffer C at pH 7.3. It was buffer at 20 ml/h flow rate for fraction volumes of 6 ml.

Figure 3. Purification of erythrocyte hemolysate GR by affinity chromatography and the columns (1.3 × 60 cm2) were eluted by buffer C at pH 7.3. It was buffer at 20 ml/h flow rate for fraction volumes of 6 ml.

Figure 3. Purification of erythrocyte hemolysate GR by affinity chromatography and the columns (1.3 × 60 cm2) were eluted by buffer C at pH 7.3. It was buffer at 20 ml/h flow rate for fraction volumes of 6 ml.

Figure 4. Optimal pH for liver and erythrocyte GR.

Figure 4. Optimal pH for liver and erythrocyte GR.

Figure 5. Optimal temperature for liver and erythrocyte GR.

Figure 5. Optimal temperature for liver and erythrocyte GR.

Figure 6. Activity–ionic strength for liver and erythrocyte GR.

Figure 6. Activity–ionic strength for liver and erythrocyte GR.

Table 1. Ki and IC50 values obtained from regression analysis graphs for glutathione reductase in the presence of different drugs concentration.

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