HPLC-DAD phenolic profile, cytotoxic and anti-kinetoplastidae activity of Melissa officinalis

Figures & data

Table 1. Leishmanicide and trypanocide activities of Melissa officinalis.

Natural productLC50 value (μg/mL)	μg/mL	% inhibition (L. infantum)105.78	% inhibition (L. brasiliensis)110.69	% inhibition (T. cruzi) 245.23
M. officinalis	25012562.531.25	80.39 ± 3.1154.27 ± 2.250 ± 2.840 ± 0.34	80.59 ± 0.0868.61 ± 0.099.87 ± 3.40 ± 2.81	54.45 ± 1.3027.26 ± 1.457.43 ± 1.322.87 ± 0.64

The results are expressed as mean ± SD (standard deviation) of n =3 independent experiments.

Table 2. Cytotoxic activity of Melissa officinalis.

Compound LC50 (μg/mL)	μg/mL	% Anti-fibroblast 141.01
	1000	87.86 ± 0.49
	500	95.41 ± 0.84
EEC3	250	73.93 ± 1.68
	125	51.26 ± 0.74

The results are expressed as mean ± SD of n = 3 independent experiments.

Figure 1. Representative high-performance liquid chromatography profile of Melissa officinalis. Gallic acid (peak 1), catechin (peak 2), chlorogenic acid (peak 3), caffeic acid (peak 4), rosmarinic acid (peak 5), ellagic acid (peak 6), epicatechin (peak 7), rutin (peak 8), isoquercitrin (peak 9), quercitrin (peak 10), quercetin (peak 11) and kaempferol (12). TR, retention time.

Table 3. Phenolics and flavonoids composition of Melissa officinalis leaves extract.

Compounds	M. officinalis		LOD (μg/mL)	LOQ (μg/mL)
	mg/g	%
Gallic acid	37.62 ± 0.01 a	3.76	0.025	0.083
Catechin	17.90 ± 0.02 b	1.79	0.013	0.042
Chlorogenic acid	9.52 ± 0.01 c	0.95	0.032	0.106
Caffeic acid	64.25 ± 0.03 d	6.42	0.016	0.052
Rosmarinic acid	22.13 ± 0.01 e	2.21	0.028	0.096
Ellagic acid	48.56 ± 0.03 f	4.85	0.007	0.023
Epicatechin	23.47 ± 0.01 e	2.34	0.021	0.074
Rutin	92.61 ± 0.02 g	9.26	0.019	0.067
Isoquercitrin	23.58 ± 0.03 e	2.35	0.012	0.039
Quercitrin	35.91 ± 0.01 a	3.59	0.035	0.113
Quercetin	41.37 ± 0.01 h	4.13	0.009	0.027
Kaempferol	9.26 ± 0.02 c	0.92	0.041	0.135

Results are expressed as mean ± standard deviations (SD) of three determinations. Averages followed by different letters differ by Tukey test at p < 0.05. LOD, limit of detection; LOQ, limit of quantification.