Figures & data
Figure 1. Effects of formononetin on CNE2 cell proliferation. CNE2 cell numbers were significantly increased by low concentrations of formononetin (0.05, 0.1, 0.2, 0.5, 1, 2 and 5 μM) treatment. OD, optical density. *p < 0.05 versus control, **p < 0.01 versus control; n = 11.
Figure 2. Effects of formononetin on the late apoptosis of CNE2 cells. 0.3 μM formononetin significantly inhibited the late apoptosis of CNE2 cells. (A) Control group, (B) 0.1 μM formononetin group, (C) 0.3 μM formononetin group, (D) 1 μM formononetin group and (E) rate of late apoptosis in CNE2 cells. *p < 0.05 versus control; n = 3.
Figure 3. Effects of formononetin on mRNA expression of bcl-2 and bax in CNE2 cells. Bcl-2 mRNA expression was significantly upregulated by 0.1 and 0.2 μM formononetin, while bax mRNA expression was significantly downregulated by 0.2 and 1 μM formononetin. **p < 0.01 versus control; n = 4.
Figure 4. Effect of formononetin on the protein expression of bcl-2 and p-ERK1/2 in CNE2 cells. (A) Formononetin increased the expression levels of bcl-2 protein, β-actin was used as the loading control. *p < 0.05 versus control; n = 3. (B) Formononetin elevated the expression levels of p-ERK1/2 protein, t-ERK1/2 was used as the loading control. *p < 0.05 versus control; n = 4.
