Sedative effect of central administration of Coriandrum sativum essential oil and its major component linalool in neonatal chicks

Figures & data

Figure 1. Chromatogram of coriander seed essential oil. Peak number indicates different compounds (Table 1).

Table 1. Composition of coriander seed essential oil (CEO).

Peak	Rt^a (min)	Compound	KIe^b	KIr^c	%^d	CEO (μl/mL)
1	10.91	α-Pinene	931	931	5.5 ± 0,4	55
2	11.66	Camphene	944	944	0.43 ± 0.02	4.3
3	13.27	β-Pinene	973	969	0.41 ± 0.05	4.1
4	14.36	Myrcene	992	993	0.58 ± 0.05	5.8
5	16.35	o-Cimene	1023	1022	0.79 ± 0.02	7.9
6	16.58	Limonene	1027	1025	0.7 ± 0.1	7
7	18.64	γ-Terpinene	1057	1056	5.7 ± 0.1	57
8	21.73	Linalool	1103	1101	81.7 ± 0.9	817
9	24.37	Camphor	1141	1143	3.1 ± 0.4	31
10	28.65	Dodecane	1201	1200	0.18 ± 0.02	1.8
11	41.33	Geranyl-acetate	1387	1383	0.68 ± 0.08	6.8
12	42.28	Tetradecane	1401	1400	0.34 ± 0.02	3.4

^aRetention time in minutes (GC-MS analysis).

^bExperimental Kovat's retention index.

^cReference Kovat's retention index.

^dRelative percentage ± standard deviation determined on triplicate analysis in GC-FID analyses.

Figure 2. Effect of i.c.v. administration of CEO on the number of vocalizations on OF in 4–6-d-old chicks. Bars represent median (interquartile range). *p < 0.05 compared with saline (Dunn's post hoc test).

Table 2. Effect of i.c.v. CEO injection on the behavioural patterns of chicks exposed to OF test for 10 min.

	Saline (n = 15)	Diazepam (n = 14)	CEO 0.86 (n = 16)	CEO 8.6 (n = 14)	CEO 86 (n = 16)
Number of squares crossed	154 (100–226)	3 (0–16.5)*	107.5 (14.5–146.5)	7.5 (4–15.5)*	5.5 (1–12)*
Attempt to escape	8 (4–12)	0 (0–0)*	5 (0–12)	0 (0–0)*	0 (0–0)*
Number of defecations	2 (1–2)	0 (0–1)*	2 (1–2.5)	1 (0–1)*	1 (0–1)*
Sleeping posture (s)	0 (0–0)	160 (64–223)*	0 (0–41)	35 (0–133.5)	140.5 (99.5–188)*

Values are expressed as median (interquartile range).

* p < 0.05 significantly different from saline and CEO 0.86 groups (Dunn's post hoc test).

Figure 3. Effect of i.c.v. administration of linalool on the number of vocalizations on OF in 4–6-d-old chicks. Bars represent median (interquartile range). *p < 0.05 compared with saline (Dunn's post hoc test).

Table 3. Effect of i.c.v. linalool injection on behavioural patterns of chicks exposed to OF test for 10 min.

	Saline (n = 15)	Diazepam (n = 14)	Linalool 0.86 (n = 15)	Linalool 8.6 (n = 14)	Linalool 86 (n = 13)
Number of squares crossed	123 (45–182)	3 (0–19)*	72 (12–100)	9 (3–18.5)*	7 (3–11)*
Attempt to escape	6 (1–13)	0 (0–0)*	1 (0–4)	0 (0–0)*	0 (0–0)*
Number of defecations	1 (1–2)	0.5 (0–1)*	1 (1–2)	0 (0–1)*	1 (0–1)*
Sleeping posture (s)	0 (0–0)	144 (37–239)*	0 (0–38)	104.5 (32.5–159.5)*	160 (6–347.5)*

Values are expressed as median (interquartile range).

* p < 0.05 significantly different from saline and Linalool 0.86 groups (Dunn's post hoc test).