Tissue hypoxaemia causes oedema, inflammation and fibrosis in porcine bronchial transsection

Figures & data

Figure 1. Bronchial mucosa blood flow (BMBF) with laser-Doppler velocimetry. Left panel: Left BMBF in study and control group. Right panel: Left/right BMBF ratio in study and control group. Filled circle: study group. Open circle: control group.

Table I. Overview of results.

		Preoperative			Postoperative			Sectioning
Group	Unit	Left	Right	L/R-ratio	Left	Right	L/R-ratio	Left	Right	L/R-ratio
Bronchial mucosa blood flow (BMBF)
Control group (n = 6)	PU/s (95%-CI)	161 (118–203)	146 (114–179)	1.11 (0.87–1.35)	205 (154–256)	176 (149–203)	1.24 (0.82–1.66)	206 (162–250)	171 (125–217)	1.32 (0.82–1.82)
Study group (n = 6)	PU/s (95%-CI)	210 (170–251)	152 (112–193)	1.44 (1.19–1.69)	115 (89–141)	184 (164–203)	0.63 (0.49–0.76)	187 (124–251)	183 (115–251)	1.02 (0.73–1.31)
Bronchial mucosa haemoglobin saturation
Control group (n = 6)	Sat (%) (95%-CI)	81 (76–87)	88 (87–90)	0.92 (0.85–0.99)	76 (64–87)	82 (75–89)	0.92 (0.82–1.03)	75 (56–95)	76 (66–86)	0.98 (0.75–1.21)
Study group (n = 6)	Sat (%) (95%-CI)	92 (82–102)	83 (74–91)	1.12 (1.05–1.19)	61 (46–77)	77 (70–83)	0.81 (0.59–1.02)	74 (61–87)	73 (62–85)	1.00 (0.88–1.12)
Bronchial mucosa haemoglobin concentration
Control group (n = 6)	mM (95%-CI)	0.32 (0.20–0.44)	0.23 (0.17–0.30)	1.45 (1.00–1.89)	0.43 (0.35–0.52)	0.40 (0.25–0.54)	1.35 (0.74–1.97)	0.35 (0.25–0.45)	0.29 (0.18–0.40)	1.42 (0.77–2.07)
Study group (n = 6)	mM (95%-CI)	0.24 (0.17–0.30)	0.22 (0.13–0.30)	1.15 (0.91–1.39)	0.45 (0.31–0.59)	0.39 (0.29–0.49)	1.18 (0.89–1.47)	0.29 (0.13–0.46)	0.34 (0.22–0.45)	0.92 (0.48–1.35)

Figure 2. Bronchial mucosa haemoglobin saturation with DRS. Left panel: Bronchial mucosa saturation of left bronchus in study and control group. Right panel: Left/right ratio of bronchial mucosa saturation in study and control group. Filled circle: study group. Open circle: control group.

Figure 3. Bronchial mucosa haemoglobin concentration with DRS. Left panel: Bronchial mucosa con-centration of left bronchus in study and control group. Right panel: Left/right ratio of bronchial mucosa concentration in study and control group. Filled circle: study group. Open circle: control group.

Figure 4. Section of left lung with major bronchi (H&E, × 100), showing severe broadening of lung septae with inflammation, oedema and slight fibrosis. Specimen was taken peripheral to both anastomosis and measuring site. Arrow: Small bronchus.